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A DNA extraction protocol for improved DNA yield from individual mosquitoes

Typical DNA extraction protocols from commercially available kits provide an adequate amount of DNA from a single individual mosquito sufficient for PCR-based assays. However, next-generation sequencing applications and high-throughput SNP genotyping assays exposed the limitation of DNA quantity one...

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Detalles Bibliográficos
Autores principales: Nieman, Catelyn C., Yamasaki, Youki, Collier, Travis C., Lee, Yoosook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743141/
https://www.ncbi.nlm.nih.gov/pubmed/26937269
http://dx.doi.org/10.12688/f1000research.7413.1