Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model

Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling suc...

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Autores principales: Yano, Shuya, Takehara, Kiyoto, Miwa, Shinji, Kishimoto, Hiroyuki, Hiroshima, Yukihiko, Murakami, Takashi, Urata, Yasuo, Kagawa, Shunsuke, Bouvet, Michael, Fujiwara, Toshiyoshi, Hoffman, Robert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743860/
https://www.ncbi.nlm.nih.gov/pubmed/26849435
http://dx.doi.org/10.1371/journal.pone.0148760
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author Yano, Shuya
Takehara, Kiyoto
Miwa, Shinji
Kishimoto, Hiroyuki
Hiroshima, Yukihiko
Murakami, Takashi
Urata, Yasuo
Kagawa, Shunsuke
Bouvet, Michael
Fujiwara, Toshiyoshi
Hoffman, Robert M.
author_facet Yano, Shuya
Takehara, Kiyoto
Miwa, Shinji
Kishimoto, Hiroyuki
Hiroshima, Yukihiko
Murakami, Takashi
Urata, Yasuo
Kagawa, Shunsuke
Bouvet, Michael
Fujiwara, Toshiyoshi
Hoffman, Robert M.
author_sort Yano, Shuya
collection PubMed
description Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS.
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spelling pubmed-47438602016-02-11 Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model Yano, Shuya Takehara, Kiyoto Miwa, Shinji Kishimoto, Hiroyuki Hiroshima, Yukihiko Murakami, Takashi Urata, Yasuo Kagawa, Shunsuke Bouvet, Michael Fujiwara, Toshiyoshi Hoffman, Robert M. PLoS One Research Article Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS. Public Library of Science 2016-02-05 /pmc/articles/PMC4743860/ /pubmed/26849435 http://dx.doi.org/10.1371/journal.pone.0148760 Text en © 2016 Yano et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yano, Shuya
Takehara, Kiyoto
Miwa, Shinji
Kishimoto, Hiroyuki
Hiroshima, Yukihiko
Murakami, Takashi
Urata, Yasuo
Kagawa, Shunsuke
Bouvet, Michael
Fujiwara, Toshiyoshi
Hoffman, Robert M.
Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
title Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
title_full Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
title_fullStr Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
title_full_unstemmed Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
title_short Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model
title_sort improved resection and outcome of colon-cancer liver metastasis with fluorescence-guided surgery using in situ gfp labeling with a telomerase-dependent adenovirus in an orthotopic mouse model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743860/
https://www.ncbi.nlm.nih.gov/pubmed/26849435
http://dx.doi.org/10.1371/journal.pone.0148760
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