Cargando…

The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects

The fermentation performance of Saccharomyces cerevisiae in the cellulose to ethanol conversion process is largely influenced by the components of pretreated biomass. The insoluble solids in pretreated biomass predominantly constitute cellulose, lignin, and -to a lesser extent- hemicellulose. It is...

Descripción completa

Detalles Bibliográficos
Autores principales: Koppram, Rakesh, Mapelli, Valeria, Albers, Eva, Olsson, Lisbeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743953/
https://www.ncbi.nlm.nih.gov/pubmed/26849651
http://dx.doi.org/10.1371/journal.pone.0148635
_version_ 1782414415563325440
author Koppram, Rakesh
Mapelli, Valeria
Albers, Eva
Olsson, Lisbeth
author_facet Koppram, Rakesh
Mapelli, Valeria
Albers, Eva
Olsson, Lisbeth
author_sort Koppram, Rakesh
collection PubMed
description The fermentation performance of Saccharomyces cerevisiae in the cellulose to ethanol conversion process is largely influenced by the components of pretreated biomass. The insoluble solids in pretreated biomass predominantly constitute cellulose, lignin, and -to a lesser extent- hemicellulose. It is important to understand the effects of water-insoluble solids (WIS) on yeast cell physiology and metabolism for the overall process optimization. In the presence of synthetic lignocellulosic inhibitors, we observed a reduced lag phase and enhanced volumetric ethanol productivity by S. cerevisiae CEN.PK 113-7D when the minimal medium was supplemented with WIS of pretreated birch or spruce and glucose as the carbon source. To investigate the underlying molecular reasons for the effects of WIS, we studied the response of WIS at the proteome level in yeast cells in the presence of acetic acid as an inhibitor. Comparisons were made with cells grown in the presence of acetic acid but without WIS in the medium. Altogether, 729 proteins were detected and quantified, of which 246 proteins were significantly up-regulated and 274 proteins were significantly down-regulated with a fold change≥1.2 in the presence of WIS compared to absence of WIS. The cells in the presence of WIS up-regulated several proteins related to cell wall, glycolysis, electron transport chain, oxidative stress response, oxygen and radical detoxification and unfolded protein response; and down-regulated most proteins related to biosynthetic pathways including amino acid, purine, isoprenoid biosynthesis, aminoacyl-tRNA synthetases and pentose phosphate pathway. Overall, the identified differentially regulated proteins may indicate that the likelihood of increased ATP generation in the presence of WIS was used to defend against acetic acid stress at the expense of reduced biomass formation. Although, comparative proteomics of cells with and without WIS in the acetic acid containing medium revealed numerous changes, a direct effect of WIS on cellular physiology remains to be investigated.
format Online
Article
Text
id pubmed-4743953
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47439532016-02-11 The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects Koppram, Rakesh Mapelli, Valeria Albers, Eva Olsson, Lisbeth PLoS One Research Article The fermentation performance of Saccharomyces cerevisiae in the cellulose to ethanol conversion process is largely influenced by the components of pretreated biomass. The insoluble solids in pretreated biomass predominantly constitute cellulose, lignin, and -to a lesser extent- hemicellulose. It is important to understand the effects of water-insoluble solids (WIS) on yeast cell physiology and metabolism for the overall process optimization. In the presence of synthetic lignocellulosic inhibitors, we observed a reduced lag phase and enhanced volumetric ethanol productivity by S. cerevisiae CEN.PK 113-7D when the minimal medium was supplemented with WIS of pretreated birch or spruce and glucose as the carbon source. To investigate the underlying molecular reasons for the effects of WIS, we studied the response of WIS at the proteome level in yeast cells in the presence of acetic acid as an inhibitor. Comparisons were made with cells grown in the presence of acetic acid but without WIS in the medium. Altogether, 729 proteins were detected and quantified, of which 246 proteins were significantly up-regulated and 274 proteins were significantly down-regulated with a fold change≥1.2 in the presence of WIS compared to absence of WIS. The cells in the presence of WIS up-regulated several proteins related to cell wall, glycolysis, electron transport chain, oxidative stress response, oxygen and radical detoxification and unfolded protein response; and down-regulated most proteins related to biosynthetic pathways including amino acid, purine, isoprenoid biosynthesis, aminoacyl-tRNA synthetases and pentose phosphate pathway. Overall, the identified differentially regulated proteins may indicate that the likelihood of increased ATP generation in the presence of WIS was used to defend against acetic acid stress at the expense of reduced biomass formation. Although, comparative proteomics of cells with and without WIS in the acetic acid containing medium revealed numerous changes, a direct effect of WIS on cellular physiology remains to be investigated. Public Library of Science 2016-02-05 /pmc/articles/PMC4743953/ /pubmed/26849651 http://dx.doi.org/10.1371/journal.pone.0148635 Text en © 2016 Koppram et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Koppram, Rakesh
Mapelli, Valeria
Albers, Eva
Olsson, Lisbeth
The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects
title The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects
title_full The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects
title_fullStr The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects
title_full_unstemmed The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects
title_short The Presence of Pretreated Lignocellulosic Solids from Birch during Saccharomyces cerevisiae Fermentations Leads to Increased Tolerance to Inhibitors – A Proteomic Study of the Effects
title_sort presence of pretreated lignocellulosic solids from birch during saccharomyces cerevisiae fermentations leads to increased tolerance to inhibitors – a proteomic study of the effects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743953/
https://www.ncbi.nlm.nih.gov/pubmed/26849651
http://dx.doi.org/10.1371/journal.pone.0148635
work_keys_str_mv AT koppramrakesh thepresenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects
AT mapellivaleria thepresenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects
AT alberseva thepresenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects
AT olssonlisbeth thepresenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects
AT koppramrakesh presenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects
AT mapellivaleria presenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects
AT alberseva presenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects
AT olssonlisbeth presenceofpretreatedlignocellulosicsolidsfrombirchduringsaccharomycescerevisiaefermentationsleadstoincreasedtolerancetoinhibitorsaproteomicstudyoftheeffects