Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm

BACKGROUND: Double injection of blood into cisterna magna using a rabbit model results in cerebral vasospasm. An unacceptably high mortality rate tends to limit the application of model. Ultrasound guided puncture can provide real-time imaging guidance for operation. The aim of this paper is to esta...

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Autores principales: Chen, Yongchao, Zhu, Youzhi, Zhang, Yu, Zhang, Zixuan, Lian, Juan, Luo, Fucheng, Deng, Xuefei, Wong, Kelvin KL
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744401/
https://www.ncbi.nlm.nih.gov/pubmed/26851937
http://dx.doi.org/10.1186/s12938-016-0123-z
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author Chen, Yongchao
Zhu, Youzhi
Zhang, Yu
Zhang, Zixuan
Lian, Juan
Luo, Fucheng
Deng, Xuefei
Wong, Kelvin KL
author_facet Chen, Yongchao
Zhu, Youzhi
Zhang, Yu
Zhang, Zixuan
Lian, Juan
Luo, Fucheng
Deng, Xuefei
Wong, Kelvin KL
author_sort Chen, Yongchao
collection PubMed
description BACKGROUND: Double injection of blood into cisterna magna using a rabbit model results in cerebral vasospasm. An unacceptably high mortality rate tends to limit the application of model. Ultrasound guided puncture can provide real-time imaging guidance for operation. The aim of this paper is to establish a safe and effective rabbit model of cerebral vasospasm after subarachnoid hemorrhage with the assistance of ultrasound medical imaging. METHODS: A total of 160 New Zealand white rabbits were randomly divided into four groups of 40 each: (1) manual control group, (2) manual model group, (3) ultrasound guided control group, and (4) ultrasound guided model group. The subarachnoid hemorrhage was intentionally caused by double injection of blood into their cisterna magna. Then, basilar artery diameters were measured using magnetic resonance angiography before modeling and 5 days after modeling. RESULTS: The depth of needle entering into cisterna magna was determined during the process of ultrasound guided puncture. The mortality rates in manual control group and model group were 15 and 23 %, respectively. No rabbits were sacrificed in those two ultrasound guided groups. We found that the mortality rate in ultrasound guided groups decreased significantly compared to manual groups. Compared with diameters before modeling, the basilar artery diameters after modeling were significantly lower in manual and ultrasound guided model groups. The vasospasm aggravated and the proportion of severe vasospasms was greater in ultrasound guided model group than that of manual group. In manual model group, no vasospasm was found in 8 % of rabbits. CONCLUSIONS: The ultrasound guided double injection of blood into cisterna magna is a safe and effective rabbit model for treatment of cerebral vasospasm.
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spelling pubmed-47444012016-02-07 Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm Chen, Yongchao Zhu, Youzhi Zhang, Yu Zhang, Zixuan Lian, Juan Luo, Fucheng Deng, Xuefei Wong, Kelvin KL Biomed Eng Online Research BACKGROUND: Double injection of blood into cisterna magna using a rabbit model results in cerebral vasospasm. An unacceptably high mortality rate tends to limit the application of model. Ultrasound guided puncture can provide real-time imaging guidance for operation. The aim of this paper is to establish a safe and effective rabbit model of cerebral vasospasm after subarachnoid hemorrhage with the assistance of ultrasound medical imaging. METHODS: A total of 160 New Zealand white rabbits were randomly divided into four groups of 40 each: (1) manual control group, (2) manual model group, (3) ultrasound guided control group, and (4) ultrasound guided model group. The subarachnoid hemorrhage was intentionally caused by double injection of blood into their cisterna magna. Then, basilar artery diameters were measured using magnetic resonance angiography before modeling and 5 days after modeling. RESULTS: The depth of needle entering into cisterna magna was determined during the process of ultrasound guided puncture. The mortality rates in manual control group and model group were 15 and 23 %, respectively. No rabbits were sacrificed in those two ultrasound guided groups. We found that the mortality rate in ultrasound guided groups decreased significantly compared to manual groups. Compared with diameters before modeling, the basilar artery diameters after modeling were significantly lower in manual and ultrasound guided model groups. The vasospasm aggravated and the proportion of severe vasospasms was greater in ultrasound guided model group than that of manual group. In manual model group, no vasospasm was found in 8 % of rabbits. CONCLUSIONS: The ultrasound guided double injection of blood into cisterna magna is a safe and effective rabbit model for treatment of cerebral vasospasm. BioMed Central 2016-02-06 /pmc/articles/PMC4744401/ /pubmed/26851937 http://dx.doi.org/10.1186/s12938-016-0123-z Text en © Chen et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Chen, Yongchao
Zhu, Youzhi
Zhang, Yu
Zhang, Zixuan
Lian, Juan
Luo, Fucheng
Deng, Xuefei
Wong, Kelvin KL
Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm
title Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm
title_full Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm
title_fullStr Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm
title_full_unstemmed Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm
title_short Ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm
title_sort ultrasound guided double injection of blood into cisterna magna: a rabbit model for treatment of cerebral vasospasm
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744401/
https://www.ncbi.nlm.nih.gov/pubmed/26851937
http://dx.doi.org/10.1186/s12938-016-0123-z
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