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Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration
Constructs intended for bone tissue engineering (TE) are influenced by the initial cell seeding density. Therefore, the objective of this study was to determine the effect of bone marrow stromal stem cells (BMSCs) density loaded onto copolymer scaffolds on bone regeneration. BMSCs were harvested fro...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744655/ https://www.ncbi.nlm.nih.gov/pubmed/26013960 http://dx.doi.org/10.1002/jbm.a.35505 |
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author | Yassin, Mohammed A. Leknes, Knut N. Pedersen, Torbjorn O. Xing, Zhe Sun, Yang Lie, Stein A. Finne‐Wistrand, Anna Mustafa, Kamal |
author_facet | Yassin, Mohammed A. Leknes, Knut N. Pedersen, Torbjorn O. Xing, Zhe Sun, Yang Lie, Stein A. Finne‐Wistrand, Anna Mustafa, Kamal |
author_sort | Yassin, Mohammed A. |
collection | PubMed |
description | Constructs intended for bone tissue engineering (TE) are influenced by the initial cell seeding density. Therefore, the objective of this study was to determine the effect of bone marrow stromal stem cells (BMSCs) density loaded onto copolymer scaffolds on bone regeneration. BMSCs were harvested from rat's bone marrow and cultured in media with or without osteogenic supplements. Cells were seeded onto poly(l‐lactide‐co‐ε‐caprolactone) [poly(LLA‐co‐CL)] scaffolds at two different densities: low density (1 × 10(6) cells/scaffold) or high density (2 × 10(6) cells/scaffold) using spinner modified flasks and examined after 1 and 3 weeks. Initial attachment and spread of BMSC onto the scaffolds was recorded by scanning electron microscopy. Cell proliferation was assessed by DNA quantification and cell differentiation by quantitative real‐time reverse transcriptase‐polymerized chain reaction analysis (qRT‐PCR). Five‐millimeter rat calvarial defects (24 defects in 12 rats) were implanted with scaffolds seeded with either low or high density expanded with or without osteogenic supplements. Osteogenic supplements significantly increased cell proliferation (p < 0.001). Scaffolds seeded at high cell density exhibited higher mRNA expressions of Runx2 p = 0.001, Col1 p = 0.001, BMP2 p < 0.001, BSP p < 0.001, and OC p = 0.013. More bone was formed in response to high cell seeding density (p = 0.023) and high seeding density with osteogenic medium (p = 0.038). Poly (LLA‐co‐CL) scaffolds could be appropriate candidates for bone TE. The optimal number of cells to be loaded onto scaffolds is critical for promoting Extracellular matrix synthesis and bone formation. Cell seeding density and osteogenic supplements may have a synergistic effect on the induction of new bone. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3649–3658, 2015. |
format | Online Article Text |
id | pubmed-4744655 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47446552016-02-18 Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration Yassin, Mohammed A. Leknes, Knut N. Pedersen, Torbjorn O. Xing, Zhe Sun, Yang Lie, Stein A. Finne‐Wistrand, Anna Mustafa, Kamal J Biomed Mater Res A Original Articles Constructs intended for bone tissue engineering (TE) are influenced by the initial cell seeding density. Therefore, the objective of this study was to determine the effect of bone marrow stromal stem cells (BMSCs) density loaded onto copolymer scaffolds on bone regeneration. BMSCs were harvested from rat's bone marrow and cultured in media with or without osteogenic supplements. Cells were seeded onto poly(l‐lactide‐co‐ε‐caprolactone) [poly(LLA‐co‐CL)] scaffolds at two different densities: low density (1 × 10(6) cells/scaffold) or high density (2 × 10(6) cells/scaffold) using spinner modified flasks and examined after 1 and 3 weeks. Initial attachment and spread of BMSC onto the scaffolds was recorded by scanning electron microscopy. Cell proliferation was assessed by DNA quantification and cell differentiation by quantitative real‐time reverse transcriptase‐polymerized chain reaction analysis (qRT‐PCR). Five‐millimeter rat calvarial defects (24 defects in 12 rats) were implanted with scaffolds seeded with either low or high density expanded with or without osteogenic supplements. Osteogenic supplements significantly increased cell proliferation (p < 0.001). Scaffolds seeded at high cell density exhibited higher mRNA expressions of Runx2 p = 0.001, Col1 p = 0.001, BMP2 p < 0.001, BSP p < 0.001, and OC p = 0.013. More bone was formed in response to high cell seeding density (p = 0.023) and high seeding density with osteogenic medium (p = 0.038). Poly (LLA‐co‐CL) scaffolds could be appropriate candidates for bone TE. The optimal number of cells to be loaded onto scaffolds is critical for promoting Extracellular matrix synthesis and bone formation. Cell seeding density and osteogenic supplements may have a synergistic effect on the induction of new bone. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3649–3658, 2015. John Wiley and Sons Inc. 2015-09-04 2015-11 /pmc/articles/PMC4744655/ /pubmed/26013960 http://dx.doi.org/10.1002/jbm.a.35505 Text en © 2015 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Yassin, Mohammed A. Leknes, Knut N. Pedersen, Torbjorn O. Xing, Zhe Sun, Yang Lie, Stein A. Finne‐Wistrand, Anna Mustafa, Kamal Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration |
title | Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration |
title_full | Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration |
title_fullStr | Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration |
title_full_unstemmed | Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration |
title_short | Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration |
title_sort | cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744655/ https://www.ncbi.nlm.nih.gov/pubmed/26013960 http://dx.doi.org/10.1002/jbm.a.35505 |
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