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Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments
The gutless marine worm O lavius algarvensis lives in symbiosis with chemosynthetic bacteria that provide nutrition by fixing carbon dioxide (CO (2)) into biomass using reduced sulfur compounds as energy sources. A recent metaproteomic analysis of the O . algarvensis symbiosis indicated that carbon...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744751/ https://www.ncbi.nlm.nih.gov/pubmed/26013766 http://dx.doi.org/10.1111/1462-2920.12912 |
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author | Kleiner, Manuel Wentrup, Cecilia Holler, Thomas Lavik, Gaute Harder, Jens Lott, Christian Littmann, Sten Kuypers, Marcel M. M. Dubilier, Nicole |
author_facet | Kleiner, Manuel Wentrup, Cecilia Holler, Thomas Lavik, Gaute Harder, Jens Lott, Christian Littmann, Sten Kuypers, Marcel M. M. Dubilier, Nicole |
author_sort | Kleiner, Manuel |
collection | PubMed |
description | The gutless marine worm O lavius algarvensis lives in symbiosis with chemosynthetic bacteria that provide nutrition by fixing carbon dioxide (CO (2)) into biomass using reduced sulfur compounds as energy sources. A recent metaproteomic analysis of the O . algarvensis symbiosis indicated that carbon monoxide (CO) and hydrogen (H (2)) might also be used as energy sources. We provide direct evidence that the O . algarvensis symbiosis consumes CO and H (2). Single cell imaging using nanoscale secondary ion mass spectrometry revealed that one of the symbionts, the γ3‐symbiont, uses the energy from CO oxidation to fix CO (2). Pore water analysis revealed considerable in‐situ concentrations of CO and H (2) in the O . algarvensis environment, Mediterranean seagrass sediments. Pore water H (2) concentrations (89–2147 nM) were up to two orders of magnitude higher than in seawater, and up to 36‐fold higher than previously known from shallow‐water marine sediments. Pore water CO concentrations (17–51 nM) were twice as high as in the overlying seawater (no literature data from other shallow‐water sediments are available for comparison). Ex‐situ incubation experiments showed that dead seagrass rhizomes produced large amounts of CO. CO production from decaying plant material could thus be a significant energy source for microbial primary production in seagrass sediments. |
format | Online Article Text |
id | pubmed-4744751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47447512016-02-18 Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments Kleiner, Manuel Wentrup, Cecilia Holler, Thomas Lavik, Gaute Harder, Jens Lott, Christian Littmann, Sten Kuypers, Marcel M. M. Dubilier, Nicole Environ Microbiol Research Articles The gutless marine worm O lavius algarvensis lives in symbiosis with chemosynthetic bacteria that provide nutrition by fixing carbon dioxide (CO (2)) into biomass using reduced sulfur compounds as energy sources. A recent metaproteomic analysis of the O . algarvensis symbiosis indicated that carbon monoxide (CO) and hydrogen (H (2)) might also be used as energy sources. We provide direct evidence that the O . algarvensis symbiosis consumes CO and H (2). Single cell imaging using nanoscale secondary ion mass spectrometry revealed that one of the symbionts, the γ3‐symbiont, uses the energy from CO oxidation to fix CO (2). Pore water analysis revealed considerable in‐situ concentrations of CO and H (2) in the O . algarvensis environment, Mediterranean seagrass sediments. Pore water H (2) concentrations (89–2147 nM) were up to two orders of magnitude higher than in seawater, and up to 36‐fold higher than previously known from shallow‐water marine sediments. Pore water CO concentrations (17–51 nM) were twice as high as in the overlying seawater (no literature data from other shallow‐water sediments are available for comparison). Ex‐situ incubation experiments showed that dead seagrass rhizomes produced large amounts of CO. CO production from decaying plant material could thus be a significant energy source for microbial primary production in seagrass sediments. John Wiley and Sons Inc. 2015-07-23 2015-12 /pmc/articles/PMC4744751/ /pubmed/26013766 http://dx.doi.org/10.1111/1462-2920.12912 Text en © 2015 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Kleiner, Manuel Wentrup, Cecilia Holler, Thomas Lavik, Gaute Harder, Jens Lott, Christian Littmann, Sten Kuypers, Marcel M. M. Dubilier, Nicole Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments |
title | Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments |
title_full | Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments |
title_fullStr | Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments |
title_full_unstemmed | Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments |
title_short | Use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments |
title_sort | use of carbon monoxide and hydrogen by a bacteria–animal symbiosis from seagrass sediments |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744751/ https://www.ncbi.nlm.nih.gov/pubmed/26013766 http://dx.doi.org/10.1111/1462-2920.12912 |
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