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Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage

Thiomicrospira were originally characterized as sulfur-oxidizing chemolithoautotrophs. Attempts to grow them on hydrogen failed for many years. Only recently we demonstrated hydrogen consumption among two of three tested Thiomicrospira and posited that hydrogen consumption may be more widespread amo...

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Autores principales: Hansen, Moritz, Perner, Mirjam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744846/
https://www.ncbi.nlm.nih.gov/pubmed/26903978
http://dx.doi.org/10.3389/fmicb.2016.00099
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author Hansen, Moritz
Perner, Mirjam
author_facet Hansen, Moritz
Perner, Mirjam
author_sort Hansen, Moritz
collection PubMed
description Thiomicrospira were originally characterized as sulfur-oxidizing chemolithoautotrophs. Attempts to grow them on hydrogen failed for many years. Only recently we demonstrated hydrogen consumption among two of three tested Thiomicrospira and posited that hydrogen consumption may be more widespread among Thiomicrospira than previously assumed. Here, we investigate and compare the hydrogen consumption ability and the presence of group 1 [NiFe]-hydrogenase genes (enzyme catalyzes H(2)↔2H(+) + 2e(-)) for sixteen different Thiomicrospira species. Seven of these Thiomicrospira species encoded group 1 [NiFe]-hydrogenase genes and five of these species could also consume hydrogen. All Thiomicrospira species exhibiting hydrogen consumption were from hydrothermal vents along the Mid-Atlantic ridge or Eastern Pacific ridges. The tested Thiomicrospira from Mediterranean and Western Pacific vents could not consume hydrogen. The [NiFe]-hydrogenase genes were categorized into two clusters: those resembling the hydrogenase from Hydrogenovibrio are in cluster I and are related to those from Alpha- and other Gammaproteobacteria. In cluster II, hydrogenases found exclusively in Thiomicrospira crunogena strains are combined and form a monophyletic group with those from Epsilonproteobacteria suggesting they were acquired through horizontal gene transfer. Hydrogen consumption appears to be common among some Thiomicrospira, given that five of the tested sixteen strains carried this trait. The hydrogen consumption ability expands their competitiveness within an environment.
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spelling pubmed-47448462016-02-22 Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage Hansen, Moritz Perner, Mirjam Front Microbiol Microbiology Thiomicrospira were originally characterized as sulfur-oxidizing chemolithoautotrophs. Attempts to grow them on hydrogen failed for many years. Only recently we demonstrated hydrogen consumption among two of three tested Thiomicrospira and posited that hydrogen consumption may be more widespread among Thiomicrospira than previously assumed. Here, we investigate and compare the hydrogen consumption ability and the presence of group 1 [NiFe]-hydrogenase genes (enzyme catalyzes H(2)↔2H(+) + 2e(-)) for sixteen different Thiomicrospira species. Seven of these Thiomicrospira species encoded group 1 [NiFe]-hydrogenase genes and five of these species could also consume hydrogen. All Thiomicrospira species exhibiting hydrogen consumption were from hydrothermal vents along the Mid-Atlantic ridge or Eastern Pacific ridges. The tested Thiomicrospira from Mediterranean and Western Pacific vents could not consume hydrogen. The [NiFe]-hydrogenase genes were categorized into two clusters: those resembling the hydrogenase from Hydrogenovibrio are in cluster I and are related to those from Alpha- and other Gammaproteobacteria. In cluster II, hydrogenases found exclusively in Thiomicrospira crunogena strains are combined and form a monophyletic group with those from Epsilonproteobacteria suggesting they were acquired through horizontal gene transfer. Hydrogen consumption appears to be common among some Thiomicrospira, given that five of the tested sixteen strains carried this trait. The hydrogen consumption ability expands their competitiveness within an environment. Frontiers Media S.A. 2016-02-08 /pmc/articles/PMC4744846/ /pubmed/26903978 http://dx.doi.org/10.3389/fmicb.2016.00099 Text en Copyright © 2016 Hansen and Perner. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hansen, Moritz
Perner, Mirjam
Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage
title Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage
title_full Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage
title_fullStr Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage
title_full_unstemmed Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage
title_short Hydrogenase Gene Distribution and H(2) Consumption Ability within the Thiomicrospira Lineage
title_sort hydrogenase gene distribution and h(2) consumption ability within the thiomicrospira lineage
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744846/
https://www.ncbi.nlm.nih.gov/pubmed/26903978
http://dx.doi.org/10.3389/fmicb.2016.00099
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