Cargando…
Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model
Introduction: There are few reports of in vivo muscle strength measurements in animal models of ICU‐acquired weakness (ICU‐AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU‐AW model using in vivo strength measurements and myo...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744984/ https://www.ncbi.nlm.nih.gov/pubmed/26015329 http://dx.doi.org/10.1002/mus.24711 |
_version_ | 1782414558508351488 |
---|---|
author | Witteveen, Esther Hoogland, Inge C.M. Wieske, Luuk Weber, Nina C. Verhamme, Camiel Schultz, Marcus J. van Schaik, Ivo N. Horn, Janneke |
author_facet | Witteveen, Esther Hoogland, Inge C.M. Wieske, Luuk Weber, Nina C. Verhamme, Camiel Schultz, Marcus J. van Schaik, Ivo N. Horn, Janneke |
author_sort | Witteveen, Esther |
collection | PubMed |
description | Introduction: There are few reports of in vivo muscle strength measurements in animal models of ICU‐acquired weakness (ICU‐AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU‐AW model using in vivo strength measurements and myosin/actin assays, and whether development of ICU‐AW is age‐dependent in this model. Methods: Young and old mice were injected intraperitoneally with E. coli and treated with ceftriaxone. Forelimb grip strength was measured at multiple time points, and the myosin/actin ratio in muscle was determined. Results: E. coli administration was not associated with grip strength decrease, neither in young nor in old mice. In old mice, the myosin/actin ratio was lower in E. coli mice at t = 48 h and higher at t = 72 h compared with controls. Conclusions: This E. coli septic peritonitis mouse model did not induce decreased grip strength. In its current form, it seems unsuitable as a model for ICU‐AW. Muscle Nerve 53: 127–133, 2016 |
format | Online Article Text |
id | pubmed-4744984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47449842016-02-18 Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model Witteveen, Esther Hoogland, Inge C.M. Wieske, Luuk Weber, Nina C. Verhamme, Camiel Schultz, Marcus J. van Schaik, Ivo N. Horn, Janneke Muscle Nerve Basic & Translational Science Introduction: There are few reports of in vivo muscle strength measurements in animal models of ICU‐acquired weakness (ICU‐AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU‐AW model using in vivo strength measurements and myosin/actin assays, and whether development of ICU‐AW is age‐dependent in this model. Methods: Young and old mice were injected intraperitoneally with E. coli and treated with ceftriaxone. Forelimb grip strength was measured at multiple time points, and the myosin/actin ratio in muscle was determined. Results: E. coli administration was not associated with grip strength decrease, neither in young nor in old mice. In old mice, the myosin/actin ratio was lower in E. coli mice at t = 48 h and higher at t = 72 h compared with controls. Conclusions: This E. coli septic peritonitis mouse model did not induce decreased grip strength. In its current form, it seems unsuitable as a model for ICU‐AW. Muscle Nerve 53: 127–133, 2016 John Wiley and Sons Inc. 2015-11-21 2016-01 /pmc/articles/PMC4744984/ /pubmed/26015329 http://dx.doi.org/10.1002/mus.24711 Text en © 2015 The Authors. Muscle & Nerve Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Basic & Translational Science Witteveen, Esther Hoogland, Inge C.M. Wieske, Luuk Weber, Nina C. Verhamme, Camiel Schultz, Marcus J. van Schaik, Ivo N. Horn, Janneke Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model |
title | Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model |
title_full | Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model |
title_fullStr | Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model |
title_full_unstemmed | Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model |
title_short | Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model |
title_sort | assessment of intensive care unit‐acquired weakness in young and old mice: an e. coli septic peritonitis model |
topic | Basic & Translational Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744984/ https://www.ncbi.nlm.nih.gov/pubmed/26015329 http://dx.doi.org/10.1002/mus.24711 |
work_keys_str_mv | AT witteveenesther assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel AT hooglandingecm assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel AT wieskeluuk assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel AT weberninac assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel AT verhammecamiel assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel AT schultzmarcusj assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel AT vanschaikivon assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel AT hornjanneke assessmentofintensivecareunitacquiredweaknessinyoungandoldmiceanecolisepticperitonitismodel |