Cargando…

An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging

Advances in neurotechnology have been integral to the investigation of neural circuit function in systems neuroscience. Recent improvements in high performance fluorescent sensors and scientific CMOS cameras enables optical imaging of neural networks at a much larger scale. While exciting technical...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohammed, Ali I., Gritton, Howard J., Tseng, Hua-an, Bucklin, Mark E., Yao, Zhaojie, Han, Xue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745097/
https://www.ncbi.nlm.nih.gov/pubmed/26854041
http://dx.doi.org/10.1038/srep20986
_version_ 1782414580999258112
author Mohammed, Ali I.
Gritton, Howard J.
Tseng, Hua-an
Bucklin, Mark E.
Yao, Zhaojie
Han, Xue
author_facet Mohammed, Ali I.
Gritton, Howard J.
Tseng, Hua-an
Bucklin, Mark E.
Yao, Zhaojie
Han, Xue
author_sort Mohammed, Ali I.
collection PubMed
description Advances in neurotechnology have been integral to the investigation of neural circuit function in systems neuroscience. Recent improvements in high performance fluorescent sensors and scientific CMOS cameras enables optical imaging of neural networks at a much larger scale. While exciting technical advances demonstrate the potential of this technique, further improvement in data acquisition and analysis, especially those that allow effective processing of increasingly larger datasets, would greatly promote the application of optical imaging in systems neuroscience. Here we demonstrate the ability of wide-field imaging to capture the concurrent dynamic activity from hundreds to thousands of neurons over millimeters of brain tissue in behaving mice. This system allows the visualization of morphological details at a higher spatial resolution than has been previously achieved using similar functional imaging modalities. To analyze the expansive data sets, we developed software to facilitate rapid downstream data processing. Using this system, we show that a large fraction of anatomically distinct hippocampal neurons respond to discrete environmental stimuli associated with classical conditioning, and that the observed temporal dynamics of transient calcium signals are sufficient for exploring certain spatiotemporal features of large neural networks.
format Online
Article
Text
id pubmed-4745097
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-47450972016-02-16 An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging Mohammed, Ali I. Gritton, Howard J. Tseng, Hua-an Bucklin, Mark E. Yao, Zhaojie Han, Xue Sci Rep Article Advances in neurotechnology have been integral to the investigation of neural circuit function in systems neuroscience. Recent improvements in high performance fluorescent sensors and scientific CMOS cameras enables optical imaging of neural networks at a much larger scale. While exciting technical advances demonstrate the potential of this technique, further improvement in data acquisition and analysis, especially those that allow effective processing of increasingly larger datasets, would greatly promote the application of optical imaging in systems neuroscience. Here we demonstrate the ability of wide-field imaging to capture the concurrent dynamic activity from hundreds to thousands of neurons over millimeters of brain tissue in behaving mice. This system allows the visualization of morphological details at a higher spatial resolution than has been previously achieved using similar functional imaging modalities. To analyze the expansive data sets, we developed software to facilitate rapid downstream data processing. Using this system, we show that a large fraction of anatomically distinct hippocampal neurons respond to discrete environmental stimuli associated with classical conditioning, and that the observed temporal dynamics of transient calcium signals are sufficient for exploring certain spatiotemporal features of large neural networks. Nature Publishing Group 2016-02-08 /pmc/articles/PMC4745097/ /pubmed/26854041 http://dx.doi.org/10.1038/srep20986 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mohammed, Ali I.
Gritton, Howard J.
Tseng, Hua-an
Bucklin, Mark E.
Yao, Zhaojie
Han, Xue
An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging
title An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging
title_full An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging
title_fullStr An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging
title_full_unstemmed An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging
title_short An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging
title_sort integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745097/
https://www.ncbi.nlm.nih.gov/pubmed/26854041
http://dx.doi.org/10.1038/srep20986
work_keys_str_mv AT mohammedalii anintegrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT grittonhowardj anintegrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT tsenghuaan anintegrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT bucklinmarke anintegrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT yaozhaojie anintegrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT hanxue anintegrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT mohammedalii integrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT grittonhowardj integrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT tsenghuaan integrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT bucklinmarke integrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT yaozhaojie integrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging
AT hanxue integrativeapproachforanalyzinghundredsofneuronsintaskperformingmiceusingwidefieldcalciumimaging