Cargando…

Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker

Bladder cancer (BCa) is one of the most common urothelial cancers with still noticeable incidence rate. Early detection of BCa is highly correlated with successful therapeutic outcomes. We previously showed that apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) was expressed at an inc...

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, Sunga, Shin, Ju Hyun, Lee, Yu Ran, Joo, Hee Kyoung, Song, Ki Hak, Na, Yong Gil, Chang, Seok Jong, Lim, Jae Sung, Jeon, Byeong Hwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745317/
https://www.ncbi.nlm.nih.gov/pubmed/27057081
http://dx.doi.org/10.1155/2016/7276502
_version_ 1782414622371872768
author Choi, Sunga
Shin, Ju Hyun
Lee, Yu Ran
Joo, Hee Kyoung
Song, Ki Hak
Na, Yong Gil
Chang, Seok Jong
Lim, Jae Sung
Jeon, Byeong Hwa
author_facet Choi, Sunga
Shin, Ju Hyun
Lee, Yu Ran
Joo, Hee Kyoung
Song, Ki Hak
Na, Yong Gil
Chang, Seok Jong
Lim, Jae Sung
Jeon, Byeong Hwa
author_sort Choi, Sunga
collection PubMed
description Bladder cancer (BCa) is one of the most common urothelial cancers with still noticeable incidence rate. Early detection of BCa is highly correlated with successful therapeutic outcomes. We previously showed that apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) was expressed at an increased level in the serum of BCa patients when compared to the level in healthy controls. In this study, we investigated whether urinary APE1/Ref-1 was also elevated in patients with BCa. In this case-control study, voided urine was collected from 277 subjects including 169 BCa patients and 108 non-BCa controls. Urinary APE1/Ref-1 level was assessed by enzyme-linked immunosorbent assay (ELISA). APE1/Ref-1 levels were significantly elevated in BCa patients relative to levels in non-BCa controls and were correlated with tumor grade and stage. Urinary APE1/Ref-1 levels were also higher in patients with recurrence history of BCa. The receiver operating characteristics (ROC) curve of APE1/Ref-1 showed an area under the curve of 0.83, indicating the reliability and validity of this biomarker. The optimal combination of sensitivity and specificity was determined to be 82% and 80% at a cut-off value of 0.376 ng/100 μL for detection of APE1/Ref-1 in urine. In conclusion, urinary APE1/Ref-1 levels measured from noninvasively obtained body fluids would be clinically applicable for diagnosis of BCa.
format Online
Article
Text
id pubmed-4745317
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-47453172016-04-07 Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker Choi, Sunga Shin, Ju Hyun Lee, Yu Ran Joo, Hee Kyoung Song, Ki Hak Na, Yong Gil Chang, Seok Jong Lim, Jae Sung Jeon, Byeong Hwa Dis Markers Research Article Bladder cancer (BCa) is one of the most common urothelial cancers with still noticeable incidence rate. Early detection of BCa is highly correlated with successful therapeutic outcomes. We previously showed that apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) was expressed at an increased level in the serum of BCa patients when compared to the level in healthy controls. In this study, we investigated whether urinary APE1/Ref-1 was also elevated in patients with BCa. In this case-control study, voided urine was collected from 277 subjects including 169 BCa patients and 108 non-BCa controls. Urinary APE1/Ref-1 level was assessed by enzyme-linked immunosorbent assay (ELISA). APE1/Ref-1 levels were significantly elevated in BCa patients relative to levels in non-BCa controls and were correlated with tumor grade and stage. Urinary APE1/Ref-1 levels were also higher in patients with recurrence history of BCa. The receiver operating characteristics (ROC) curve of APE1/Ref-1 showed an area under the curve of 0.83, indicating the reliability and validity of this biomarker. The optimal combination of sensitivity and specificity was determined to be 82% and 80% at a cut-off value of 0.376 ng/100 μL for detection of APE1/Ref-1 in urine. In conclusion, urinary APE1/Ref-1 levels measured from noninvasively obtained body fluids would be clinically applicable for diagnosis of BCa. Hindawi Publishing Corporation 2016 2016-01-21 /pmc/articles/PMC4745317/ /pubmed/27057081 http://dx.doi.org/10.1155/2016/7276502 Text en Copyright © 2016 Sunga Choi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Choi, Sunga
Shin, Ju Hyun
Lee, Yu Ran
Joo, Hee Kyoung
Song, Ki Hak
Na, Yong Gil
Chang, Seok Jong
Lim, Jae Sung
Jeon, Byeong Hwa
Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker
title Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker
title_full Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker
title_fullStr Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker
title_full_unstemmed Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker
title_short Urinary APE1/Ref-1: A Potential Bladder Cancer Biomarker
title_sort urinary ape1/ref-1: a potential bladder cancer biomarker
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745317/
https://www.ncbi.nlm.nih.gov/pubmed/27057081
http://dx.doi.org/10.1155/2016/7276502
work_keys_str_mv AT choisunga urinaryape1ref1apotentialbladdercancerbiomarker
AT shinjuhyun urinaryape1ref1apotentialbladdercancerbiomarker
AT leeyuran urinaryape1ref1apotentialbladdercancerbiomarker
AT jooheekyoung urinaryape1ref1apotentialbladdercancerbiomarker
AT songkihak urinaryape1ref1apotentialbladdercancerbiomarker
AT nayonggil urinaryape1ref1apotentialbladdercancerbiomarker
AT changseokjong urinaryape1ref1apotentialbladdercancerbiomarker
AT limjaesung urinaryape1ref1apotentialbladdercancerbiomarker
AT jeonbyeonghwa urinaryape1ref1apotentialbladdercancerbiomarker