Cargando…
MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway
Wnt/β-catenin signaling pathway is strictly controlled by multiple negative regulators. However, how tumor cells override the negative regulatory effects to maintain constitutive activation of Wnt/β-catenin signaling, which is commonly observed in various cancers, remains puzzling. In current study,...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745698/ https://www.ncbi.nlm.nih.gov/pubmed/26337084 |
_version_ | 1782414698094788608 |
---|---|
author | Wu, Geyan Liu, Aibin Zhu, Jinrong Lei, Fangyong Wu, Shu Zhang, Xin Ye, Liping Cao, Lixue He, Shanyang |
author_facet | Wu, Geyan Liu, Aibin Zhu, Jinrong Lei, Fangyong Wu, Shu Zhang, Xin Ye, Liping Cao, Lixue He, Shanyang |
author_sort | Wu, Geyan |
collection | PubMed |
description | Wnt/β-catenin signaling pathway is strictly controlled by multiple negative regulators. However, how tumor cells override the negative regulatory effects to maintain constitutive activation of Wnt/β-catenin signaling, which is commonly observed in various cancers, remains puzzling. In current study, we reported that overexpression of miR-1207 in ovarian cancer activated Wnt/β-catenin signaling by directly targeting and suppressing secreted Frizzled-related protein 1 (SFRP1), AXIN2 and inhibitor of β-catenin and TCF-4 (ICAT), which are vital negative regulators of the Wnt/β-catenin pathway. We found that the expression of miR-1207 was ubiquitously upregulated in both ovarian cancer tissues and cells, which inversely correlated with patient overall survival. Furthermore, overexpression of miR-1207 enhanced, while silencing miR-1207 reduced, stem cell-like traits of ovarian cancer cells in vitro and in vivo, including tumor sphere formation capability and proportion of SP+ and CD133+ cells. Importantly, upregulating miR-1207 promoted, while silencing miR-1207 inhibited, the tumorigenicity of ovarian cancer cells. Hence, our results suggest that miR-1207 plays a vital role in promoting the cancer stem cell-like phenotype in ovarian cancer and might represent a potential target for anti-ovarian cancer therapy. |
format | Online Article Text |
id | pubmed-4745698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-47456982016-02-23 MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway Wu, Geyan Liu, Aibin Zhu, Jinrong Lei, Fangyong Wu, Shu Zhang, Xin Ye, Liping Cao, Lixue He, Shanyang Oncotarget Research Paper Wnt/β-catenin signaling pathway is strictly controlled by multiple negative regulators. However, how tumor cells override the negative regulatory effects to maintain constitutive activation of Wnt/β-catenin signaling, which is commonly observed in various cancers, remains puzzling. In current study, we reported that overexpression of miR-1207 in ovarian cancer activated Wnt/β-catenin signaling by directly targeting and suppressing secreted Frizzled-related protein 1 (SFRP1), AXIN2 and inhibitor of β-catenin and TCF-4 (ICAT), which are vital negative regulators of the Wnt/β-catenin pathway. We found that the expression of miR-1207 was ubiquitously upregulated in both ovarian cancer tissues and cells, which inversely correlated with patient overall survival. Furthermore, overexpression of miR-1207 enhanced, while silencing miR-1207 reduced, stem cell-like traits of ovarian cancer cells in vitro and in vivo, including tumor sphere formation capability and proportion of SP+ and CD133+ cells. Importantly, upregulating miR-1207 promoted, while silencing miR-1207 inhibited, the tumorigenicity of ovarian cancer cells. Hence, our results suggest that miR-1207 plays a vital role in promoting the cancer stem cell-like phenotype in ovarian cancer and might represent a potential target for anti-ovarian cancer therapy. Impact Journals LLC 2015-08-17 /pmc/articles/PMC4745698/ /pubmed/26337084 Text en Copyright: © 2015 Wu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wu, Geyan Liu, Aibin Zhu, Jinrong Lei, Fangyong Wu, Shu Zhang, Xin Ye, Liping Cao, Lixue He, Shanyang MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway |
title | MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway |
title_full | MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway |
title_fullStr | MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway |
title_full_unstemmed | MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway |
title_short | MiR-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the Wnt/β-catenin signaling pathway |
title_sort | mir-1207 overexpression promotes cancer stem cell–like traits in ovarian cancer by activating the wnt/β-catenin signaling pathway |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745698/ https://www.ncbi.nlm.nih.gov/pubmed/26337084 |
work_keys_str_mv | AT wugeyan mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT liuaibin mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT zhujinrong mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT leifangyong mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT wushu mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT zhangxin mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT yeliping mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT caolixue mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway AT heshanyang mir1207overexpressionpromotescancerstemcellliketraitsinovariancancerbyactivatingthewntbcateninsignalingpathway |