Cargando…

Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2

Reactive oxygen species (ROS) are closely related to the follicular granulosa cell apoptosis. Grape seed procyanidin B2 (GSPB2) has been reported to possess potent antioxidant activity. However, the GSPB2-mediated protective effects and the underlying molecular mechanisms in granulosa cell apoptosis...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Jia-Qing, Gao, Bin-Wen, Wang, Jing, Ren, Qiao-Ling, Chen, Jun-Feng, Ma, Qiang, Zhang, Zi-Jing, Xing, Bao-Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745910/
https://www.ncbi.nlm.nih.gov/pubmed/27057282
http://dx.doi.org/10.1155/2016/6147345
_version_ 1782414739019661312
author Zhang, Jia-Qing
Gao, Bin-Wen
Wang, Jing
Ren, Qiao-Ling
Chen, Jun-Feng
Ma, Qiang
Zhang, Zi-Jing
Xing, Bao-Song
author_facet Zhang, Jia-Qing
Gao, Bin-Wen
Wang, Jing
Ren, Qiao-Ling
Chen, Jun-Feng
Ma, Qiang
Zhang, Zi-Jing
Xing, Bao-Song
author_sort Zhang, Jia-Qing
collection PubMed
description Reactive oxygen species (ROS) are closely related to the follicular granulosa cell apoptosis. Grape seed procyanidin B2 (GSPB2) has been reported to possess potent antioxidant activity. However, the GSPB2-mediated protective effects and the underlying molecular mechanisms in granulosa cell apoptosis process remain unknown. In this study, we showed for the first time that GSPB2 treatment decreased FoxO1 protein level, improved granulosa cell viability, upregulated LC3-II protein level, and reduced granulosa cell apoptosis rate. Under a condition of oxidative stress, GSPB2 reversed FoxO1 nuclear localization and increased its level in cytoplasm. In addition, FoxO1 knockdown inhibited the protective effects of GSPB2 induced. Our findings suggest that FoxO1 plays a pivotal role in regulating autophagy in granulosa cells, GSPB2 exerts a potent and beneficial role in reducing granulosa cell apoptosis and inducing autophagy process, and targeting FoxO1 could be significant in fighting against oxidative stress-reduced female reproductive system diseases.
format Online
Article
Text
id pubmed-4745910
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-47459102016-04-07 Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2 Zhang, Jia-Qing Gao, Bin-Wen Wang, Jing Ren, Qiao-Ling Chen, Jun-Feng Ma, Qiang Zhang, Zi-Jing Xing, Bao-Song Oxid Med Cell Longev Research Article Reactive oxygen species (ROS) are closely related to the follicular granulosa cell apoptosis. Grape seed procyanidin B2 (GSPB2) has been reported to possess potent antioxidant activity. However, the GSPB2-mediated protective effects and the underlying molecular mechanisms in granulosa cell apoptosis process remain unknown. In this study, we showed for the first time that GSPB2 treatment decreased FoxO1 protein level, improved granulosa cell viability, upregulated LC3-II protein level, and reduced granulosa cell apoptosis rate. Under a condition of oxidative stress, GSPB2 reversed FoxO1 nuclear localization and increased its level in cytoplasm. In addition, FoxO1 knockdown inhibited the protective effects of GSPB2 induced. Our findings suggest that FoxO1 plays a pivotal role in regulating autophagy in granulosa cells, GSPB2 exerts a potent and beneficial role in reducing granulosa cell apoptosis and inducing autophagy process, and targeting FoxO1 could be significant in fighting against oxidative stress-reduced female reproductive system diseases. Hindawi Publishing Corporation 2016 2016-01-19 /pmc/articles/PMC4745910/ /pubmed/27057282 http://dx.doi.org/10.1155/2016/6147345 Text en Copyright © 2016 Jia-Qing Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Jia-Qing
Gao, Bin-Wen
Wang, Jing
Ren, Qiao-Ling
Chen, Jun-Feng
Ma, Qiang
Zhang, Zi-Jing
Xing, Bao-Song
Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
title Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
title_full Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
title_fullStr Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
title_full_unstemmed Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
title_short Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
title_sort critical role of foxo1 in granulosa cell apoptosis caused by oxidative stress and protective effects of grape seed procyanidin b2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745910/
https://www.ncbi.nlm.nih.gov/pubmed/27057282
http://dx.doi.org/10.1155/2016/6147345
work_keys_str_mv AT zhangjiaqing criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2
AT gaobinwen criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2
AT wangjing criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2
AT renqiaoling criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2
AT chenjunfeng criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2
AT maqiang criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2
AT zhangzijing criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2
AT xingbaosong criticalroleoffoxo1ingranulosacellapoptosiscausedbyoxidativestressandprotectiveeffectsofgrapeseedprocyanidinb2