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Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol
PURPOSE: Clinical and epidemiological studies have indicated that breastfeeding has a protective effect on breast cancer risk. Protein-based drugs, including antibodies, are being developed to attain better forms of cancer therapy. Secretory IgA (SIgA) is the antibody class in human breast milk, and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745958/ https://www.ncbi.nlm.nih.gov/pubmed/26893571 http://dx.doi.org/10.2147/OTT.S99839 |
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author | Honorio-França, Adenilda Cristina Nunes, Gabriel Triches Fagundes, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Fernandes, Rubian Trindade da Silva França, Juliana Luzia França-Botelho, Aline do Carmo Moraes, Lucélia Campelo Albuquerque Varotti, Fernando de Pilla França, Eduardo Luzía |
author_facet | Honorio-França, Adenilda Cristina Nunes, Gabriel Triches Fagundes, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Fernandes, Rubian Trindade da Silva França, Juliana Luzia França-Botelho, Aline do Carmo Moraes, Lucélia Campelo Albuquerque Varotti, Fernando de Pilla França, Eduardo Luzía |
author_sort | Honorio-França, Adenilda Cristina |
collection | PubMed |
description | PURPOSE: Clinical and epidemiological studies have indicated that breastfeeding has a protective effect on breast cancer risk. Protein-based drugs, including antibodies, are being developed to attain better forms of cancer therapy. Secretory IgA (SIgA) is the antibody class in human breast milk, and its activity can be linked to the protective effect of breastfeeding. The aim of this study was to investigate the effect of polyethylene glycol (PEG) microspheres with adsorbed SIgA on MCF-7 human breast cancer cells. METHODS: The PEG microspheres were characterized by flow cytometry and fluorescence microscopy. The MCF-7 cells were obtained from American Type Culture Collection. MCF-7 cells were pre-incubated for 24 hours with or without SIgA (100 ng/mL), PEG microspheres or SIgA adsorbed in PEG microspheres (100 ng/mL). Viability, intracellular calcium release, and apoptosis in MCF-7 cells were determined by flow cytometry. RESULTS: Fluorescence microscopy and flow cytometry analyses revealed that SIgA was able to adsorb to the PEG microspheres. The MCF-7 cells that were incubated with PEG microspheres with adsorbed SIgA showed decreased viability. MCF-7 cells that were incubated with SIgA or PEG microspheres with adsorbed SIgA had increased intracellular Ca(2+) levels. In the presence of SIgA, an increase in the percentage of apoptotic cells was observed. The highest apoptosis index was observed when the cells were treated with PEG microspheres with adsorbed SIgA. CONCLUSION: These data suggest that colostral SIgA adsorbed to PEG microspheres has antitumor effects on human MCF-7 breast cancer cells and that the presence of large amounts of this protein in secreted breast milk may provide protection against breast tumors in women who breastfed. |
format | Online Article Text |
id | pubmed-4745958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47459582016-02-18 Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol Honorio-França, Adenilda Cristina Nunes, Gabriel Triches Fagundes, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Fernandes, Rubian Trindade da Silva França, Juliana Luzia França-Botelho, Aline do Carmo Moraes, Lucélia Campelo Albuquerque Varotti, Fernando de Pilla França, Eduardo Luzía Onco Targets Ther Original Research PURPOSE: Clinical and epidemiological studies have indicated that breastfeeding has a protective effect on breast cancer risk. Protein-based drugs, including antibodies, are being developed to attain better forms of cancer therapy. Secretory IgA (SIgA) is the antibody class in human breast milk, and its activity can be linked to the protective effect of breastfeeding. The aim of this study was to investigate the effect of polyethylene glycol (PEG) microspheres with adsorbed SIgA on MCF-7 human breast cancer cells. METHODS: The PEG microspheres were characterized by flow cytometry and fluorescence microscopy. The MCF-7 cells were obtained from American Type Culture Collection. MCF-7 cells were pre-incubated for 24 hours with or without SIgA (100 ng/mL), PEG microspheres or SIgA adsorbed in PEG microspheres (100 ng/mL). Viability, intracellular calcium release, and apoptosis in MCF-7 cells were determined by flow cytometry. RESULTS: Fluorescence microscopy and flow cytometry analyses revealed that SIgA was able to adsorb to the PEG microspheres. The MCF-7 cells that were incubated with PEG microspheres with adsorbed SIgA showed decreased viability. MCF-7 cells that were incubated with SIgA or PEG microspheres with adsorbed SIgA had increased intracellular Ca(2+) levels. In the presence of SIgA, an increase in the percentage of apoptotic cells was observed. The highest apoptosis index was observed when the cells were treated with PEG microspheres with adsorbed SIgA. CONCLUSION: These data suggest that colostral SIgA adsorbed to PEG microspheres has antitumor effects on human MCF-7 breast cancer cells and that the presence of large amounts of this protein in secreted breast milk may provide protection against breast tumors in women who breastfed. Dove Medical Press 2016-02-03 /pmc/articles/PMC4745958/ /pubmed/26893571 http://dx.doi.org/10.2147/OTT.S99839 Text en © 2016 Honorio-França et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Honorio-França, Adenilda Cristina Nunes, Gabriel Triches Fagundes, Danny Laura Gomes de Marchi, Patrícia Gelli Feres Fernandes, Rubian Trindade da Silva França, Juliana Luzia França-Botelho, Aline do Carmo Moraes, Lucélia Campelo Albuquerque Varotti, Fernando de Pilla França, Eduardo Luzía Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol |
title | Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol |
title_full | Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol |
title_fullStr | Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol |
title_full_unstemmed | Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol |
title_short | Intracellular calcium is a target of modulation of apoptosis in MCF-7 cells in the presence of IgA adsorbed to polyethylene glycol |
title_sort | intracellular calcium is a target of modulation of apoptosis in mcf-7 cells in the presence of iga adsorbed to polyethylene glycol |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745958/ https://www.ncbi.nlm.nih.gov/pubmed/26893571 http://dx.doi.org/10.2147/OTT.S99839 |
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