Cargando…
Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced
Cnaphalocrocis medinalis is a major pest of rice in South and South-East Asia. Insecticides are the major means farmers use for management. A naturally occurring baculovirus, C. medinalis granulovirus (CnmeGV), has been isolated from the larvae and this has the potential for use as microbial agent....
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746121/ https://www.ncbi.nlm.nih.gov/pubmed/26848752 http://dx.doi.org/10.1371/journal.pone.0147882 |
_version_ | 1782414763612962816 |
---|---|
author | Han, Guangjie Xu, Jian Liu, Qin Li, Chuanming Xu, Hongxing Lu, Zhongxian |
author_facet | Han, Guangjie Xu, Jian Liu, Qin Li, Chuanming Xu, Hongxing Lu, Zhongxian |
author_sort | Han, Guangjie |
collection | PubMed |
description | Cnaphalocrocis medinalis is a major pest of rice in South and South-East Asia. Insecticides are the major means farmers use for management. A naturally occurring baculovirus, C. medinalis granulovirus (CnmeGV), has been isolated from the larvae and this has the potential for use as microbial agent. Here, we described the complete genome sequence of CnmeGV and compared it to other baculovirus genomes. The genome of CnmeGV is 112,060 base pairs in length, has a G+C content of 35.2%. It contains 133 putative open reading frames (ORFs) of at least 150 nucleotides. A hundred and one (101) of these ORFs are homologous to other baculovirus genes including 37 baculovirus core genes. Thirty-two (32) ORFs are unique to CnmeGV with no homologues detected in the GeneBank and 53 tandem repeats (TRs) with sequence length from 25 to 551 nt intersperse throughout the genome of CnmeGV. Six (6) homologous regions (hrs) were identified interspersed throughout the genome. Hr2 contains 11 imperfect palindromes and a high content of AT sequence (about 73%). The unique ORF28 contains a coiled-coil region and a zinc finger-like domain of 4–50 residues specialized by two C2C2 zinc finger motifs that putatively bound two atoms of zinc. ORF21 encoding a chit-1 protein suggesting a horizontal gene transfer from alphabaculovirus. The putative protein presents two carbohydrate-binding module family 14 (CBM_14) domains rather than other homologues detected from betabaculovirus that only contains one chit-binding region. Gene synteny maps showed the colinearity of sequenced betabaculovirus. Phylogenetic analysis indicated that CnmeGV grouped in the betabaculovirus, with a close relation to AdorGV. The cladogram obtained in this work grouped the 17 complete GV genomes in one monophyletic clade. CnmeGV represents a new crambidae host-isolated virus species from the genus Betabaculovirus and is most closely relative of AdorGV. The analyses and information derived from this study will provide a better understanding of the pathological symptoms caused by this virus and its potential use as a microbial pesticide. |
format | Online Article Text |
id | pubmed-4746121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47461212016-02-11 Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced Han, Guangjie Xu, Jian Liu, Qin Li, Chuanming Xu, Hongxing Lu, Zhongxian PLoS One Research Article Cnaphalocrocis medinalis is a major pest of rice in South and South-East Asia. Insecticides are the major means farmers use for management. A naturally occurring baculovirus, C. medinalis granulovirus (CnmeGV), has been isolated from the larvae and this has the potential for use as microbial agent. Here, we described the complete genome sequence of CnmeGV and compared it to other baculovirus genomes. The genome of CnmeGV is 112,060 base pairs in length, has a G+C content of 35.2%. It contains 133 putative open reading frames (ORFs) of at least 150 nucleotides. A hundred and one (101) of these ORFs are homologous to other baculovirus genes including 37 baculovirus core genes. Thirty-two (32) ORFs are unique to CnmeGV with no homologues detected in the GeneBank and 53 tandem repeats (TRs) with sequence length from 25 to 551 nt intersperse throughout the genome of CnmeGV. Six (6) homologous regions (hrs) were identified interspersed throughout the genome. Hr2 contains 11 imperfect palindromes and a high content of AT sequence (about 73%). The unique ORF28 contains a coiled-coil region and a zinc finger-like domain of 4–50 residues specialized by two C2C2 zinc finger motifs that putatively bound two atoms of zinc. ORF21 encoding a chit-1 protein suggesting a horizontal gene transfer from alphabaculovirus. The putative protein presents two carbohydrate-binding module family 14 (CBM_14) domains rather than other homologues detected from betabaculovirus that only contains one chit-binding region. Gene synteny maps showed the colinearity of sequenced betabaculovirus. Phylogenetic analysis indicated that CnmeGV grouped in the betabaculovirus, with a close relation to AdorGV. The cladogram obtained in this work grouped the 17 complete GV genomes in one monophyletic clade. CnmeGV represents a new crambidae host-isolated virus species from the genus Betabaculovirus and is most closely relative of AdorGV. The analyses and information derived from this study will provide a better understanding of the pathological symptoms caused by this virus and its potential use as a microbial pesticide. Public Library of Science 2016-02-05 /pmc/articles/PMC4746121/ /pubmed/26848752 http://dx.doi.org/10.1371/journal.pone.0147882 Text en © 2016 Han et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Han, Guangjie Xu, Jian Liu, Qin Li, Chuanming Xu, Hongxing Lu, Zhongxian Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced |
title | Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced |
title_full | Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced |
title_fullStr | Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced |
title_full_unstemmed | Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced |
title_short | Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced |
title_sort | genome of cnaphalocrocis medinalis granulovirus, the first crambidae-infecting betabaculovirus isolated from rice leaffolder to sequenced |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746121/ https://www.ncbi.nlm.nih.gov/pubmed/26848752 http://dx.doi.org/10.1371/journal.pone.0147882 |
work_keys_str_mv | AT hanguangjie genomeofcnaphalocrocismedinalisgranulovirusthefirstcrambidaeinfectingbetabaculovirusisolatedfromriceleaffoldertosequenced AT xujian genomeofcnaphalocrocismedinalisgranulovirusthefirstcrambidaeinfectingbetabaculovirusisolatedfromriceleaffoldertosequenced AT liuqin genomeofcnaphalocrocismedinalisgranulovirusthefirstcrambidaeinfectingbetabaculovirusisolatedfromriceleaffoldertosequenced AT lichuanming genomeofcnaphalocrocismedinalisgranulovirusthefirstcrambidaeinfectingbetabaculovirusisolatedfromriceleaffoldertosequenced AT xuhongxing genomeofcnaphalocrocismedinalisgranulovirusthefirstcrambidaeinfectingbetabaculovirusisolatedfromriceleaffoldertosequenced AT luzhongxian genomeofcnaphalocrocismedinalisgranulovirusthefirstcrambidaeinfectingbetabaculovirusisolatedfromriceleaffoldertosequenced |