Cloning, expression, purification and characterization of lipase from Bacillus licheniformis, isolated from hot spring of Himachal Pradesh, India

In the present investigation, a gene encoding extracellular lipase was cloned from a Bacillus licheniformis. The recombinant protein containing His-tag was expressed as inclusion bodies in Esherichia coli BL21DE3 cells, using pET-23a as expression vector. Expressed protein purified from the inclusio...

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Detalles Bibliográficos
Autores principales: Kaur, Gagandeep, Singh, Amninder, Sharma, Rohit, Sharma, Vinay, Verma, Swati, Sharma, Pushpender K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746201/
https://www.ncbi.nlm.nih.gov/pubmed/28330118
http://dx.doi.org/10.1007/s13205-016-0369-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746201/
https://www.ncbi.nlm.nih.gov/pubmed/28330118
http://dx.doi.org/10.1007/s13205-016-0369-y

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