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New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers

Deep subsurface aquifers despite difficult access, represent important water resources and, at the same time, are key locations for subsurface engineering activities for the oil and gas industries, geothermal energy, and CO(2) or energy storage. Formation water originating from a 760 m-deep geologic...

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Autores principales: Aüllo, Thomas, Berlendis, Sabrina, Lascourrèges, Jean-François, Dessort, Daniel, Duclerc, Dominique, Saint-Laurent, Stéphanie, Schraauwers, Blandine, Mas, Johan, Patriarche, Delphine, Boesinger, Cécile, Magot, Michel, Ranchou-Peyruse, Anthony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746249/
https://www.ncbi.nlm.nih.gov/pubmed/26904000
http://dx.doi.org/10.3389/fmicb.2016.00122
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author Aüllo, Thomas
Berlendis, Sabrina
Lascourrèges, Jean-François
Dessort, Daniel
Duclerc, Dominique
Saint-Laurent, Stéphanie
Schraauwers, Blandine
Mas, Johan
Patriarche, Delphine
Boesinger, Cécile
Magot, Michel
Ranchou-Peyruse, Anthony
author_facet Aüllo, Thomas
Berlendis, Sabrina
Lascourrèges, Jean-François
Dessort, Daniel
Duclerc, Dominique
Saint-Laurent, Stéphanie
Schraauwers, Blandine
Mas, Johan
Patriarche, Delphine
Boesinger, Cécile
Magot, Michel
Ranchou-Peyruse, Anthony
author_sort Aüllo, Thomas
collection PubMed
description Deep subsurface aquifers despite difficult access, represent important water resources and, at the same time, are key locations for subsurface engineering activities for the oil and gas industries, geothermal energy, and CO(2) or energy storage. Formation water originating from a 760 m-deep geological gas storage aquifer was sampled and microcosms were set up to test the biodegradation potential of BTEX by indigenous microorganisms. The microbial community diversity was studied using molecular approaches based on 16S rRNA genes. After a long incubation period, with several subcultures, a sulfate-reducing consortium composed of only two Desulfotomaculum populations was observed able to degrade benzene, toluene, and ethylbenzene, extending the number of hydrocarbonoclastic–related species among the Desulfotomaculum genus. Furthermore, we were able to couple specific carbon and hydrogen isotopic fractionation during benzene removal and the results obtained by dual compound specific isotope analysis (𝜀(C) = -2.4‰ ± 0.3‰; 𝜀(H) = -57‰ ± 0.98‰; AKIE(C): 1.0146 ± 0.0009, and AKIE(H): 1.5184 ± 0.0283) were close to those obtained previously in sulfate-reducing conditions: this finding could confirm the existence of a common enzymatic reaction involving sulfate-reducers to activate benzene anaerobically. Although we cannot assign the role of each population of Desulfotomaculum in the mono-aromatic hydrocarbon degradation, this study suggests an important role of the genus Desulfotomaculum as potential biodegrader among indigenous populations in subsurface habitats. This community represents the simplest model of benzene-degrading anaerobes originating from the deepest subterranean settings ever described. As Desulfotomaculum species are often encountered in subsurface environments, this study provides some interesting results for assessing the natural response of these specific hydrologic systems in response to BTEX contamination during remediation projects.
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spelling pubmed-47462492016-02-22 New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers Aüllo, Thomas Berlendis, Sabrina Lascourrèges, Jean-François Dessort, Daniel Duclerc, Dominique Saint-Laurent, Stéphanie Schraauwers, Blandine Mas, Johan Patriarche, Delphine Boesinger, Cécile Magot, Michel Ranchou-Peyruse, Anthony Front Microbiol Microbiology Deep subsurface aquifers despite difficult access, represent important water resources and, at the same time, are key locations for subsurface engineering activities for the oil and gas industries, geothermal energy, and CO(2) or energy storage. Formation water originating from a 760 m-deep geological gas storage aquifer was sampled and microcosms were set up to test the biodegradation potential of BTEX by indigenous microorganisms. The microbial community diversity was studied using molecular approaches based on 16S rRNA genes. After a long incubation period, with several subcultures, a sulfate-reducing consortium composed of only two Desulfotomaculum populations was observed able to degrade benzene, toluene, and ethylbenzene, extending the number of hydrocarbonoclastic–related species among the Desulfotomaculum genus. Furthermore, we were able to couple specific carbon and hydrogen isotopic fractionation during benzene removal and the results obtained by dual compound specific isotope analysis (𝜀(C) = -2.4‰ ± 0.3‰; 𝜀(H) = -57‰ ± 0.98‰; AKIE(C): 1.0146 ± 0.0009, and AKIE(H): 1.5184 ± 0.0283) were close to those obtained previously in sulfate-reducing conditions: this finding could confirm the existence of a common enzymatic reaction involving sulfate-reducers to activate benzene anaerobically. Although we cannot assign the role of each population of Desulfotomaculum in the mono-aromatic hydrocarbon degradation, this study suggests an important role of the genus Desulfotomaculum as potential biodegrader among indigenous populations in subsurface habitats. This community represents the simplest model of benzene-degrading anaerobes originating from the deepest subterranean settings ever described. As Desulfotomaculum species are often encountered in subsurface environments, this study provides some interesting results for assessing the natural response of these specific hydrologic systems in response to BTEX contamination during remediation projects. Frontiers Media S.A. 2016-02-09 /pmc/articles/PMC4746249/ /pubmed/26904000 http://dx.doi.org/10.3389/fmicb.2016.00122 Text en Copyright © 2016 Aüllo, Berlendis, Lascourrèges, Dessort, Duclerc, Saint-Laurent, Schraauwers, Mas, Patriarche, Boesinger, Magot, Ranchou-Peyruse. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Aüllo, Thomas
Berlendis, Sabrina
Lascourrèges, Jean-François
Dessort, Daniel
Duclerc, Dominique
Saint-Laurent, Stéphanie
Schraauwers, Blandine
Mas, Johan
Patriarche, Delphine
Boesinger, Cécile
Magot, Michel
Ranchou-Peyruse, Anthony
New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers
title New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers
title_full New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers
title_fullStr New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers
title_full_unstemmed New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers
title_short New Bio-Indicators for Long Term Natural Attenuation of Monoaromatic Compounds in Deep Terrestrial Aquifers
title_sort new bio-indicators for long term natural attenuation of monoaromatic compounds in deep terrestrial aquifers
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746249/
https://www.ncbi.nlm.nih.gov/pubmed/26904000
http://dx.doi.org/10.3389/fmicb.2016.00122
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