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Down-Regulatory Effects of miR-211 on Long Non-Coding RNA SOX2OT and SOX2 Genes in Esophageal Squamous Cell Carcinoma
OBJECTIVE: MicroRNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that tran- scriptionally or post-transcriptionally regulate gene expression through degradation of their mRNA targets and/or translational suppression. However, there are a few reports on miRNA-mediated expression regulation of lo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746409/ https://www.ncbi.nlm.nih.gov/pubmed/26862518 |
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author | Shafiee, Mohammad Aleyasin, Seyed Ahmad Vasei, Mohammad Semnani, Shahriar Semnani Mowla, Seyed Javad |
author_facet | Shafiee, Mohammad Aleyasin, Seyed Ahmad Vasei, Mohammad Semnani, Shahriar Semnani Mowla, Seyed Javad |
author_sort | Shafiee, Mohammad |
collection | PubMed |
description | OBJECTIVE: MicroRNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that tran- scriptionally or post-transcriptionally regulate gene expression through degradation of their mRNA targets and/or translational suppression. However, there are a few reports on miRNA-mediated expression regulation of long ncRNAs (lncRNAs). We have previ- ously reported a significant upregulation of the lncRNA SOX2OT and its intronic cod- ing gene, SOX2, in esophageal squamous cell carcinoma (ESCC) tissue samples. In this study, we aimed to evaluate the effect of induced overexpression of miR-211 on SOX2OT and SOX2 expression in vitro. MATERIALS AND METHODS: In this experimental study, we performed both bioinformatic and experimental analyses to examine whether these transcripts are regulated by miRNAs. From the list of potential candidate miRNAs, miR-211 was found to have complementary sequences to SOX2OT and SOX2 transcripts. To validate our finding experimentally, we transfected the NT-2 pluripotent cell line (an embryonal carcinoma stem cell) with an expression vector overexpressing miR-211. The expression chang- es of miR-211, SOX2OT, and SOX2 were then quantified by a real-time polymerase chain reaction (RT-PCR) approach. RESULTS: Compared with mock-transfected cells, overexpression of miR-211 caused a significant down-regulation of both genes (P<0.05). Furthermore, flow-cytometry analysis revealed a significant elevation in sub-G1 cell population following ectopic expression of miR-211 in NT-2 cells. CONCLUSION: We report here, for the first time, the down-regulation of SOX2OT and SOX2 genes by an miRNA. Considering the vital role of SOX2OT and SOX2 genes in pluripotency and tumorigenesis, our data suggest an important and inhibitory role for miR-211 in the aforementioned processes. |
format | Online Article Text |
id | pubmed-4746409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-47464092016-02-09 Down-Regulatory Effects of miR-211 on Long Non-Coding RNA SOX2OT and SOX2 Genes in Esophageal Squamous Cell Carcinoma Shafiee, Mohammad Aleyasin, Seyed Ahmad Vasei, Mohammad Semnani, Shahriar Semnani Mowla, Seyed Javad Cell J Original Article OBJECTIVE: MicroRNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that tran- scriptionally or post-transcriptionally regulate gene expression through degradation of their mRNA targets and/or translational suppression. However, there are a few reports on miRNA-mediated expression regulation of long ncRNAs (lncRNAs). We have previ- ously reported a significant upregulation of the lncRNA SOX2OT and its intronic cod- ing gene, SOX2, in esophageal squamous cell carcinoma (ESCC) tissue samples. In this study, we aimed to evaluate the effect of induced overexpression of miR-211 on SOX2OT and SOX2 expression in vitro. MATERIALS AND METHODS: In this experimental study, we performed both bioinformatic and experimental analyses to examine whether these transcripts are regulated by miRNAs. From the list of potential candidate miRNAs, miR-211 was found to have complementary sequences to SOX2OT and SOX2 transcripts. To validate our finding experimentally, we transfected the NT-2 pluripotent cell line (an embryonal carcinoma stem cell) with an expression vector overexpressing miR-211. The expression chang- es of miR-211, SOX2OT, and SOX2 were then quantified by a real-time polymerase chain reaction (RT-PCR) approach. RESULTS: Compared with mock-transfected cells, overexpression of miR-211 caused a significant down-regulation of both genes (P<0.05). Furthermore, flow-cytometry analysis revealed a significant elevation in sub-G1 cell population following ectopic expression of miR-211 in NT-2 cells. CONCLUSION: We report here, for the first time, the down-regulation of SOX2OT and SOX2 genes by an miRNA. Considering the vital role of SOX2OT and SOX2 genes in pluripotency and tumorigenesis, our data suggest an important and inhibitory role for miR-211 in the aforementioned processes. Royan Institute 2016 2016-01-17 /pmc/articles/PMC4746409/ /pubmed/26862518 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Shafiee, Mohammad Aleyasin, Seyed Ahmad Vasei, Mohammad Semnani, Shahriar Semnani Mowla, Seyed Javad Down-Regulatory Effects of miR-211 on Long Non-Coding RNA SOX2OT and SOX2 Genes in Esophageal Squamous Cell Carcinoma |
title | Down-Regulatory Effects of miR-211 on Long Non-Coding
RNA SOX2OT and SOX2 Genes in Esophageal
Squamous Cell Carcinoma |
title_full | Down-Regulatory Effects of miR-211 on Long Non-Coding
RNA SOX2OT and SOX2 Genes in Esophageal
Squamous Cell Carcinoma |
title_fullStr | Down-Regulatory Effects of miR-211 on Long Non-Coding
RNA SOX2OT and SOX2 Genes in Esophageal
Squamous Cell Carcinoma |
title_full_unstemmed | Down-Regulatory Effects of miR-211 on Long Non-Coding
RNA SOX2OT and SOX2 Genes in Esophageal
Squamous Cell Carcinoma |
title_short | Down-Regulatory Effects of miR-211 on Long Non-Coding
RNA SOX2OT and SOX2 Genes in Esophageal
Squamous Cell Carcinoma |
title_sort | down-regulatory effects of mir-211 on long non-coding
rna sox2ot and sox2 genes in esophageal
squamous cell carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746409/ https://www.ncbi.nlm.nih.gov/pubmed/26862518 |
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