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Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons

Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability to perform subcellular light stimulation to investigate localized signaling has been limited. Here, we introduce an all optical method for the...

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Autores principales: Lavoie-Cardinal, Flavie, Salesse, Charleen, Bergeron, Éric, Meunier, Michel, De Koninck, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746645/
https://www.ncbi.nlm.nih.gov/pubmed/26857748
http://dx.doi.org/10.1038/srep20619
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author Lavoie-Cardinal, Flavie
Salesse, Charleen
Bergeron, Éric
Meunier, Michel
De Koninck, Paul
author_facet Lavoie-Cardinal, Flavie
Salesse, Charleen
Bergeron, Éric
Meunier, Michel
De Koninck, Paul
author_sort Lavoie-Cardinal, Flavie
collection PubMed
description Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability to perform subcellular light stimulation to investigate localized signaling has been limited. Here, we introduce an all optical method for the stimulation and the monitoring of localized Ca(2+) signaling in neurons that takes advantage of plasmonic excitation of gold nanoparticles (AuNPs). We show with confocal microscopy that 800 nm laser pulse application onto a neuron decorated with a few AuNPs triggers a transient increase in free Ca(2+), measured optically with GCaMP6s. We show that action potentials, measured electrophysiologically, can be induced with this approach. We demonstrate activation of local Ca(2+) transients and Ca(2+) signaling via CaMKII in dendritic domains, by illuminating a single or few functionalized AuNPs specifically targeting genetically-modified neurons. This NP-Assisted Localized Optical Stimulation (NALOS) provides a new complement to light-dependent methods for controlling neuronal activity and cell signaling.
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spelling pubmed-47466452016-02-17 Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons Lavoie-Cardinal, Flavie Salesse, Charleen Bergeron, Éric Meunier, Michel De Koninck, Paul Sci Rep Article Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability to perform subcellular light stimulation to investigate localized signaling has been limited. Here, we introduce an all optical method for the stimulation and the monitoring of localized Ca(2+) signaling in neurons that takes advantage of plasmonic excitation of gold nanoparticles (AuNPs). We show with confocal microscopy that 800 nm laser pulse application onto a neuron decorated with a few AuNPs triggers a transient increase in free Ca(2+), measured optically with GCaMP6s. We show that action potentials, measured electrophysiologically, can be induced with this approach. We demonstrate activation of local Ca(2+) transients and Ca(2+) signaling via CaMKII in dendritic domains, by illuminating a single or few functionalized AuNPs specifically targeting genetically-modified neurons. This NP-Assisted Localized Optical Stimulation (NALOS) provides a new complement to light-dependent methods for controlling neuronal activity and cell signaling. Nature Publishing Group 2016-02-09 /pmc/articles/PMC4746645/ /pubmed/26857748 http://dx.doi.org/10.1038/srep20619 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lavoie-Cardinal, Flavie
Salesse, Charleen
Bergeron, Éric
Meunier, Michel
De Koninck, Paul
Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons
title Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons
title_full Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons
title_fullStr Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons
title_full_unstemmed Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons
title_short Gold nanoparticle-assisted all optical localized stimulation and monitoring of Ca(2+) signaling in neurons
title_sort gold nanoparticle-assisted all optical localized stimulation and monitoring of ca(2+) signaling in neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746645/
https://www.ncbi.nlm.nih.gov/pubmed/26857748
http://dx.doi.org/10.1038/srep20619
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