Quantifying the cooperative subunit action in a multimeric membrane receptor

In multimeric membrane receptors the cooperative action of the subunits prevents exact knowledge about the operation and the interaction of the individual subunits. We propose a method that permits quantification of ligand binding to and activation effects of the individual binding sites in a multim...

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Autores principales: Wongsamitkul, Nisa, Nache, Vasilica, Eick, Thomas, Hummert, Sabine, Schulz, Eckhard, Schmauder, Ralf, Schirmeyer, Jana, Zimmer, Thomas, Benndorf, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746656/
https://www.ncbi.nlm.nih.gov/pubmed/26858151
http://dx.doi.org/10.1038/srep20974
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author Wongsamitkul, Nisa
Nache, Vasilica
Eick, Thomas
Hummert, Sabine
Schulz, Eckhard
Schmauder, Ralf
Schirmeyer, Jana
Zimmer, Thomas
Benndorf, Klaus
author_facet Wongsamitkul, Nisa
Nache, Vasilica
Eick, Thomas
Hummert, Sabine
Schulz, Eckhard
Schmauder, Ralf
Schirmeyer, Jana
Zimmer, Thomas
Benndorf, Klaus
author_sort Wongsamitkul, Nisa
collection PubMed
description In multimeric membrane receptors the cooperative action of the subunits prevents exact knowledge about the operation and the interaction of the individual subunits. We propose a method that permits quantification of ligand binding to and activation effects of the individual binding sites in a multimeric membrane receptor. The power of this method is demonstrated by gaining detailed insight into the subunit action in olfactory cyclic nucleotide-gated CNGA2 ion channels.
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spelling pubmed-47466562016-02-17 Quantifying the cooperative subunit action in a multimeric membrane receptor Wongsamitkul, Nisa Nache, Vasilica Eick, Thomas Hummert, Sabine Schulz, Eckhard Schmauder, Ralf Schirmeyer, Jana Zimmer, Thomas Benndorf, Klaus Sci Rep Article In multimeric membrane receptors the cooperative action of the subunits prevents exact knowledge about the operation and the interaction of the individual subunits. We propose a method that permits quantification of ligand binding to and activation effects of the individual binding sites in a multimeric membrane receptor. The power of this method is demonstrated by gaining detailed insight into the subunit action in olfactory cyclic nucleotide-gated CNGA2 ion channels. Nature Publishing Group 2016-02-09 /pmc/articles/PMC4746656/ /pubmed/26858151 http://dx.doi.org/10.1038/srep20974 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wongsamitkul, Nisa
Nache, Vasilica
Eick, Thomas
Hummert, Sabine
Schulz, Eckhard
Schmauder, Ralf
Schirmeyer, Jana
Zimmer, Thomas
Benndorf, Klaus
Quantifying the cooperative subunit action in a multimeric membrane receptor
title Quantifying the cooperative subunit action in a multimeric membrane receptor
title_full Quantifying the cooperative subunit action in a multimeric membrane receptor
title_fullStr Quantifying the cooperative subunit action in a multimeric membrane receptor
title_full_unstemmed Quantifying the cooperative subunit action in a multimeric membrane receptor
title_short Quantifying the cooperative subunit action in a multimeric membrane receptor
title_sort quantifying the cooperative subunit action in a multimeric membrane receptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746656/
https://www.ncbi.nlm.nih.gov/pubmed/26858151
http://dx.doi.org/10.1038/srep20974
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