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CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation

The CRISPR/Cas9 system has recently been adapted for generating knockout mice to investigate physiological functions and pathological mechanisms. Here, we report a highly efficient procedure for brain-specific disruption of genes of interest in vivo. We constructed pX330 plasmids expressing humanize...

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Detalles Bibliográficos
Autores principales:	Shinmyo, Yohei, Tanaka, Satoshi, Tsunoda, Shinichi, Hosomichi, Kazuyoshi, Tajima, Atsushi, Kawasaki, Hiroshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746659/ https://www.ncbi.nlm.nih.gov/pubmed/26857612 http://dx.doi.org/10.1038/srep20611

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