Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae

F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the th...

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Autores principales: Xia, Pengpeng, Wang, Yiting, Zhu, Congrui, Zou, Yajie, Yang, Ying, Liu, Wei, Hardwidge, Philip R., Zhu, Guoqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746772/
https://www.ncbi.nlm.nih.gov/pubmed/26857562
http://dx.doi.org/10.1186/s13567-016-0313-5
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author Xia, Pengpeng
Wang, Yiting
Zhu, Congrui
Zou, Yajie
Yang, Ying
Liu, Wei
Hardwidge, Philip R.
Zhu, Guoqiang
author_facet Xia, Pengpeng
Wang, Yiting
Zhu, Congrui
Zou, Yajie
Yang, Ying
Liu, Wei
Hardwidge, Philip R.
Zhu, Guoqiang
author_sort Xia, Pengpeng
collection PubMed
description F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+)E. coli to intestinal epithelial cells.
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spelling pubmed-47467722016-02-10 Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae Xia, Pengpeng Wang, Yiting Zhu, Congrui Zou, Yajie Yang, Ying Liu, Wei Hardwidge, Philip R. Zhu, Guoqiang Vet Res Research Article F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+)E. coli to intestinal epithelial cells. BioMed Central 2016-02-09 2016 /pmc/articles/PMC4746772/ /pubmed/26857562 http://dx.doi.org/10.1186/s13567-016-0313-5 Text en © Xia et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Xia, Pengpeng
Wang, Yiting
Zhu, Congrui
Zou, Yajie
Yang, Ying
Liu, Wei
Hardwidge, Philip R.
Zhu, Guoqiang
Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
title Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
title_full Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
title_fullStr Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
title_full_unstemmed Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
title_short Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
title_sort porcine aminopeptidase n binds to f4(+) enterotoxigenic escherichia coli fimbriae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746772/
https://www.ncbi.nlm.nih.gov/pubmed/26857562
http://dx.doi.org/10.1186/s13567-016-0313-5
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