Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells

Recent studies show that long non-coding RNAs (lncRNAs) may be significant functional regulators in tumor development, including bladder cancer. Here, we found that PVT1 was upregulated in bladder cancer tissues and cells. Further experiments revealed that PVT1 promoted cell proliferation and suppre...

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Autores principales: Zhuang, Chengle, Li, Jianfa, Liu, Yuchen, Chen, Mingwei, Yuan, Jiancheng, Fu, Xing, Zhan, Yonghao, Liu, Li, Lin, Junhao, Zhou, Qing, Xu, Wen, Zhao, Guoping, Cai, Zhiming, Huang, Weiren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747399/
https://www.ncbi.nlm.nih.gov/pubmed/26517688
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author Zhuang, Chengle
Li, Jianfa
Liu, Yuchen
Chen, Mingwei
Yuan, Jiancheng
Fu, Xing
Zhan, Yonghao
Liu, Li
Lin, Junhao
Zhou, Qing
Xu, Wen
Zhao, Guoping
Cai, Zhiming
Huang, Weiren
author_facet Zhuang, Chengle
Li, Jianfa
Liu, Yuchen
Chen, Mingwei
Yuan, Jiancheng
Fu, Xing
Zhan, Yonghao
Liu, Li
Lin, Junhao
Zhou, Qing
Xu, Wen
Zhao, Guoping
Cai, Zhiming
Huang, Weiren
author_sort Zhuang, Chengle
collection PubMed
description Recent studies show that long non-coding RNAs (lncRNAs) may be significant functional regulators in tumor development, including bladder cancer. Here, we found that PVT1 was upregulated in bladder cancer tissues and cells. Further experiments revealed that PVT1 promoted cell proliferation and suppressed cell apoptosis. Furthermore we also used the emerging technology, synthetic biology, to create tetracycline-inducible small hairpin RNA (shRNA) vectors which silenced PVT1 in a dosage-dependent manner to inhibit the progression of bladder cancer. In conclusion, data suggest that PVT1 could be an oncogene and may be a therapeutic target in bladder cancer. Synthetic “tetracycline-on” switch system can be used to quantitatively control the expression of PVT1 in bladder cancer in response to different concentration of doxycycline to suppress the progression of bladder cancer.
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spelling pubmed-47473992016-03-24 Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells Zhuang, Chengle Li, Jianfa Liu, Yuchen Chen, Mingwei Yuan, Jiancheng Fu, Xing Zhan, Yonghao Liu, Li Lin, Junhao Zhou, Qing Xu, Wen Zhao, Guoping Cai, Zhiming Huang, Weiren Oncotarget Research Paper Recent studies show that long non-coding RNAs (lncRNAs) may be significant functional regulators in tumor development, including bladder cancer. Here, we found that PVT1 was upregulated in bladder cancer tissues and cells. Further experiments revealed that PVT1 promoted cell proliferation and suppressed cell apoptosis. Furthermore we also used the emerging technology, synthetic biology, to create tetracycline-inducible small hairpin RNA (shRNA) vectors which silenced PVT1 in a dosage-dependent manner to inhibit the progression of bladder cancer. In conclusion, data suggest that PVT1 could be an oncogene and may be a therapeutic target in bladder cancer. Synthetic “tetracycline-on” switch system can be used to quantitatively control the expression of PVT1 in bladder cancer in response to different concentration of doxycycline to suppress the progression of bladder cancer. Impact Journals LLC 2015-10-15 /pmc/articles/PMC4747399/ /pubmed/26517688 Text en Copyright: © 2015 Zhuang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhuang, Chengle
Li, Jianfa
Liu, Yuchen
Chen, Mingwei
Yuan, Jiancheng
Fu, Xing
Zhan, Yonghao
Liu, Li
Lin, Junhao
Zhou, Qing
Xu, Wen
Zhao, Guoping
Cai, Zhiming
Huang, Weiren
Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells
title Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells
title_full Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells
title_fullStr Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells
title_full_unstemmed Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells
title_short Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells
title_sort tetracycline-inducible shrna targeting long non-coding rna pvt1 inhibits cell growth and induces apoptosis in bladder cancer cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747399/
https://www.ncbi.nlm.nih.gov/pubmed/26517688
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