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Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor

Heterologeous production of rhamnolipids in Pseudomonas putida is characterized by advantages of a non-pathogenic host and avoidance of the native quorum sensing regulation in Pseudomonas aeruginosa. Yet, downstream processing is a major problem in rhamnolipid production and increases in complexity...

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Autores principales: Beuker, Janina, Steier, Anke, Wittgens, Andreas, Rosenau, Frank, Henkel, Marius, Hausmann, Rudolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747948/
https://www.ncbi.nlm.nih.gov/pubmed/26860613
http://dx.doi.org/10.1186/s13568-016-0183-2
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author Beuker, Janina
Steier, Anke
Wittgens, Andreas
Rosenau, Frank
Henkel, Marius
Hausmann, Rudolf
author_facet Beuker, Janina
Steier, Anke
Wittgens, Andreas
Rosenau, Frank
Henkel, Marius
Hausmann, Rudolf
author_sort Beuker, Janina
collection PubMed
description Heterologeous production of rhamnolipids in Pseudomonas putida is characterized by advantages of a non-pathogenic host and avoidance of the native quorum sensing regulation in Pseudomonas aeruginosa. Yet, downstream processing is a major problem in rhamnolipid production and increases in complexity at low rhamnolipid titers and when using chemical foam control. This leaves the necessity of a simple concentrating and purification method. Foam fractionation is an elegant method for in situ product removal when producing microbial surfactants. However, up to now in situ foam fractionation is nearly exclusively reported for the production of surfactin with Bacillus subtilis. So far no cultivation integrated foam fractionation process for rhamnolipid production has been reported. This is probably due to excessive bacterial foam enrichment in that system. In this article a simple integrated foam fractionation process is reported for heterologous rhamnolipid production in a bioreactor with easily manageable bacterial foam enrichments. Rhamnolipids were highly concentrated in the foam during the cultivation process with enrichment factors up to 200. The described process was evaluated at different pH, media compositions and temperatures. Foam fractionation processes were characterized by calculating procedural parameter including rhamnolipid and bacterial enrichment, rhamnolipid recovery, Y(X/S), Y(P/X), and specific as well as volumetric productivities. Comparing foam fractionation parameters of the rhamnolipid process with the surfactin process a high effectiveness of the integrated foam fractionation for rhamnolipid production was demonstrated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-016-0183-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-47479482016-02-19 Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor Beuker, Janina Steier, Anke Wittgens, Andreas Rosenau, Frank Henkel, Marius Hausmann, Rudolf AMB Express Original Article Heterologeous production of rhamnolipids in Pseudomonas putida is characterized by advantages of a non-pathogenic host and avoidance of the native quorum sensing regulation in Pseudomonas aeruginosa. Yet, downstream processing is a major problem in rhamnolipid production and increases in complexity at low rhamnolipid titers and when using chemical foam control. This leaves the necessity of a simple concentrating and purification method. Foam fractionation is an elegant method for in situ product removal when producing microbial surfactants. However, up to now in situ foam fractionation is nearly exclusively reported for the production of surfactin with Bacillus subtilis. So far no cultivation integrated foam fractionation process for rhamnolipid production has been reported. This is probably due to excessive bacterial foam enrichment in that system. In this article a simple integrated foam fractionation process is reported for heterologous rhamnolipid production in a bioreactor with easily manageable bacterial foam enrichments. Rhamnolipids were highly concentrated in the foam during the cultivation process with enrichment factors up to 200. The described process was evaluated at different pH, media compositions and temperatures. Foam fractionation processes were characterized by calculating procedural parameter including rhamnolipid and bacterial enrichment, rhamnolipid recovery, Y(X/S), Y(P/X), and specific as well as volumetric productivities. Comparing foam fractionation parameters of the rhamnolipid process with the surfactin process a high effectiveness of the integrated foam fractionation for rhamnolipid production was demonstrated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-016-0183-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-02-09 /pmc/articles/PMC4747948/ /pubmed/26860613 http://dx.doi.org/10.1186/s13568-016-0183-2 Text en © Beuker et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Beuker, Janina
Steier, Anke
Wittgens, Andreas
Rosenau, Frank
Henkel, Marius
Hausmann, Rudolf
Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor
title Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor
title_full Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor
title_fullStr Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor
title_full_unstemmed Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor
title_short Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor
title_sort integrated foam fractionation for heterologous rhamnolipid production with recombinant pseudomonas putida in a bioreactor
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747948/
https://www.ncbi.nlm.nih.gov/pubmed/26860613
http://dx.doi.org/10.1186/s13568-016-0183-2
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