φXANES: In vivo imaging of metal-protein coordination environments

We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protei...

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Autores principales: James, Simon A., Hare, Dominic J., Jenkins, Nicole L., de Jonge, Martin D., Bush, Ashley I., McColl, Gawain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748213/
https://www.ncbi.nlm.nih.gov/pubmed/26861174
http://dx.doi.org/10.1038/srep20350
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author James, Simon A.
Hare, Dominic J.
Jenkins, Nicole L.
de Jonge, Martin D.
Bush, Ashley I.
McColl, Gawain
author_facet James, Simon A.
Hare, Dominic J.
Jenkins, Nicole L.
de Jonge, Martin D.
Bush, Ashley I.
McColl, Gawain
author_sort James, Simon A.
collection PubMed
description We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.
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spelling pubmed-47482132016-02-17 φXANES: In vivo imaging of metal-protein coordination environments James, Simon A. Hare, Dominic J. Jenkins, Nicole L. de Jonge, Martin D. Bush, Ashley I. McColl, Gawain Sci Rep Article We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment. Nature Publishing Group 2016-02-10 /pmc/articles/PMC4748213/ /pubmed/26861174 http://dx.doi.org/10.1038/srep20350 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
James, Simon A.
Hare, Dominic J.
Jenkins, Nicole L.
de Jonge, Martin D.
Bush, Ashley I.
McColl, Gawain
φXANES: In vivo imaging of metal-protein coordination environments
title φXANES: In vivo imaging of metal-protein coordination environments
title_full φXANES: In vivo imaging of metal-protein coordination environments
title_fullStr φXANES: In vivo imaging of metal-protein coordination environments
title_full_unstemmed φXANES: In vivo imaging of metal-protein coordination environments
title_short φXANES: In vivo imaging of metal-protein coordination environments
title_sort φxanes: in vivo imaging of metal-protein coordination environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748213/
https://www.ncbi.nlm.nih.gov/pubmed/26861174
http://dx.doi.org/10.1038/srep20350
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AT mccollgawain phxanesinvivoimagingofmetalproteincoordinationenvironments

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