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Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development

Seed development dedicates to reserve synthesis and accumulation and uncovering its genetic and biochemical mechanisms has been a major research focus. Although proteomic and transcriptomic analyses revealed dynamic changes of genes and enzymes involved, the information regarding concomitant metabol...

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Autores principales: Hu, Chaoyang, Tohge, Takayuki, Chan, Shen-An, Song, Yue, Rao, Jun, Cui, Bo, Lin, Hong, Wang, Lei, Fernie, Alisdair R., Zhang, Dabing, Shi, Jianxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748235/
https://www.ncbi.nlm.nih.gov/pubmed/26860358
http://dx.doi.org/10.1038/srep20942
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author Hu, Chaoyang
Tohge, Takayuki
Chan, Shen-An
Song, Yue
Rao, Jun
Cui, Bo
Lin, Hong
Wang, Lei
Fernie, Alisdair R.
Zhang, Dabing
Shi, Jianxin
author_facet Hu, Chaoyang
Tohge, Takayuki
Chan, Shen-An
Song, Yue
Rao, Jun
Cui, Bo
Lin, Hong
Wang, Lei
Fernie, Alisdair R.
Zhang, Dabing
Shi, Jianxin
author_sort Hu, Chaoyang
collection PubMed
description Seed development dedicates to reserve synthesis and accumulation and uncovering its genetic and biochemical mechanisms has been a major research focus. Although proteomic and transcriptomic analyses revealed dynamic changes of genes and enzymes involved, the information regarding concomitant metabolic changes is missing. Here we investigated the dynamic metabolic changes along the rice grain development of two japonica and two indica cultivars using non-targeted metabolomics approach, in which we successfully identified 214 metabolites. Statistical analyses revealed both cultivar and developmental stage dependent metabolic changes in rice grains. Generally, the stage specific metabolic kinetics corresponded well to the physiological status of the developing grains, and metabolic changes in developing rice grain are similar to those of dicot Arabidopsis and tomato at reserve accumulation stage but are different from those of dicots at seed desiccation stage. The remarkable difference in metabolite abundances between japonica and indica rice grain was observed at the reserve accumulation stage. Metabolite-metabolite correlation analysis uncovered potential new pathways for several metabolites. Taken together, this study uncovered both conserved and diverse development associated metabolic kinetics of rice grains, which facilitates further study to explore fundamental questions regarding the evolution of seed metabolic capabilities as well as their potential applications in crop improvement.
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spelling pubmed-47482352016-02-17 Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development Hu, Chaoyang Tohge, Takayuki Chan, Shen-An Song, Yue Rao, Jun Cui, Bo Lin, Hong Wang, Lei Fernie, Alisdair R. Zhang, Dabing Shi, Jianxin Sci Rep Article Seed development dedicates to reserve synthesis and accumulation and uncovering its genetic and biochemical mechanisms has been a major research focus. Although proteomic and transcriptomic analyses revealed dynamic changes of genes and enzymes involved, the information regarding concomitant metabolic changes is missing. Here we investigated the dynamic metabolic changes along the rice grain development of two japonica and two indica cultivars using non-targeted metabolomics approach, in which we successfully identified 214 metabolites. Statistical analyses revealed both cultivar and developmental stage dependent metabolic changes in rice grains. Generally, the stage specific metabolic kinetics corresponded well to the physiological status of the developing grains, and metabolic changes in developing rice grain are similar to those of dicot Arabidopsis and tomato at reserve accumulation stage but are different from those of dicots at seed desiccation stage. The remarkable difference in metabolite abundances between japonica and indica rice grain was observed at the reserve accumulation stage. Metabolite-metabolite correlation analysis uncovered potential new pathways for several metabolites. Taken together, this study uncovered both conserved and diverse development associated metabolic kinetics of rice grains, which facilitates further study to explore fundamental questions regarding the evolution of seed metabolic capabilities as well as their potential applications in crop improvement. Nature Publishing Group 2016-02-10 /pmc/articles/PMC4748235/ /pubmed/26860358 http://dx.doi.org/10.1038/srep20942 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hu, Chaoyang
Tohge, Takayuki
Chan, Shen-An
Song, Yue
Rao, Jun
Cui, Bo
Lin, Hong
Wang, Lei
Fernie, Alisdair R.
Zhang, Dabing
Shi, Jianxin
Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development
title Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development
title_full Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development
title_fullStr Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development
title_full_unstemmed Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development
title_short Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development
title_sort identification of conserved and diverse metabolic shifts during rice grain development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748235/
https://www.ncbi.nlm.nih.gov/pubmed/26860358
http://dx.doi.org/10.1038/srep20942
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