Cargando…

Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure

Analysis of the transcriptome of peripheral blood mononuclear cells (PBMCs) from patients with hepatitis B-related acute-on-chronic liver failure (HBV-ACLF) is essential to elucidate the pathogenesis of HBV-ACLF and identify HBV-ACLF-specific biomarkers. In this study, high-throughput sequencing was...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Qian, Ding, Wenchao, Jiang, Longyan, Xin, Jiaojiao, Wu, Tianzhou, Shi, Dongyan, Jiang, Jing, Cao, Hongcui, Li, Lanjuan, Li, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748289/
https://www.ncbi.nlm.nih.gov/pubmed/26861114
http://dx.doi.org/10.1038/srep20759
_version_ 1782415100545597440
author Zhou, Qian
Ding, Wenchao
Jiang, Longyan
Xin, Jiaojiao
Wu, Tianzhou
Shi, Dongyan
Jiang, Jing
Cao, Hongcui
Li, Lanjuan
Li, Jun
author_facet Zhou, Qian
Ding, Wenchao
Jiang, Longyan
Xin, Jiaojiao
Wu, Tianzhou
Shi, Dongyan
Jiang, Jing
Cao, Hongcui
Li, Lanjuan
Li, Jun
author_sort Zhou, Qian
collection PubMed
description Analysis of the transcriptome of peripheral blood mononuclear cells (PBMCs) from patients with hepatitis B-related acute-on-chronic liver failure (HBV-ACLF) is essential to elucidate the pathogenesis of HBV-ACLF and identify HBV-ACLF-specific biomarkers. In this study, high-throughput sequencing was performed to characterize the transcriptome of PMBCs from patients with HBV-ACLF. Specifically, 2381 differentially expressed genes (DEGs) and 776 differentially expressed transcripts were identified through comparisons with patients with chronic hepatitis B (CHB) and healthy controls. Gene Ontology (GO) analysis identified 114 GO terms that were clustered into 12 groups. We merged 10 dysregulated genes selected from these grouped GO terms and non-clustered terms with four significant genes with a specificity of >0.8 in the HBV-ACLF patients to obtain a set of 13 unique genes. The quantitative real-time polymerase chain reaction (qRT-PCR) validation of the top six genes (CYP19A1, SEMA6B, INHBA, DEFT1P, AZU1 and DEFA4) was consistent with the results of messenger ribonucleic acid (mRNA) sequencing. A further receiver operating characteristic (ROC) analysis revealed that the areas under the ROC curves of the six genes were all >0.8, which indicated their significant diagnostic potentials for HBV-ACLF. Conclusion: The transcriptome characteristics of PBMCs are altered in patients with HBV-ACLF, and six genes may serve as biomarkers of HBV-ACLF.
format Online
Article
Text
id pubmed-4748289
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-47482892016-02-17 Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure Zhou, Qian Ding, Wenchao Jiang, Longyan Xin, Jiaojiao Wu, Tianzhou Shi, Dongyan Jiang, Jing Cao, Hongcui Li, Lanjuan Li, Jun Sci Rep Article Analysis of the transcriptome of peripheral blood mononuclear cells (PBMCs) from patients with hepatitis B-related acute-on-chronic liver failure (HBV-ACLF) is essential to elucidate the pathogenesis of HBV-ACLF and identify HBV-ACLF-specific biomarkers. In this study, high-throughput sequencing was performed to characterize the transcriptome of PMBCs from patients with HBV-ACLF. Specifically, 2381 differentially expressed genes (DEGs) and 776 differentially expressed transcripts were identified through comparisons with patients with chronic hepatitis B (CHB) and healthy controls. Gene Ontology (GO) analysis identified 114 GO terms that were clustered into 12 groups. We merged 10 dysregulated genes selected from these grouped GO terms and non-clustered terms with four significant genes with a specificity of >0.8 in the HBV-ACLF patients to obtain a set of 13 unique genes. The quantitative real-time polymerase chain reaction (qRT-PCR) validation of the top six genes (CYP19A1, SEMA6B, INHBA, DEFT1P, AZU1 and DEFA4) was consistent with the results of messenger ribonucleic acid (mRNA) sequencing. A further receiver operating characteristic (ROC) analysis revealed that the areas under the ROC curves of the six genes were all >0.8, which indicated their significant diagnostic potentials for HBV-ACLF. Conclusion: The transcriptome characteristics of PBMCs are altered in patients with HBV-ACLF, and six genes may serve as biomarkers of HBV-ACLF. Nature Publishing Group 2016-02-10 /pmc/articles/PMC4748289/ /pubmed/26861114 http://dx.doi.org/10.1038/srep20759 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhou, Qian
Ding, Wenchao
Jiang, Longyan
Xin, Jiaojiao
Wu, Tianzhou
Shi, Dongyan
Jiang, Jing
Cao, Hongcui
Li, Lanjuan
Li, Jun
Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure
title Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure
title_full Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure
title_fullStr Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure
title_full_unstemmed Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure
title_short Comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis B-related acute-on-chronic liver failure
title_sort comparative transcriptome analysis of peripheral blood mononuclear cells in hepatitis b-related acute-on-chronic liver failure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748289/
https://www.ncbi.nlm.nih.gov/pubmed/26861114
http://dx.doi.org/10.1038/srep20759
work_keys_str_mv AT zhouqian comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT dingwenchao comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT jianglongyan comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT xinjiaojiao comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT wutianzhou comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT shidongyan comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT jiangjing comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT caohongcui comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT lilanjuan comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure
AT lijun comparativetranscriptomeanalysisofperipheralbloodmononuclearcellsinhepatitisbrelatedacuteonchronicliverfailure