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Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line

BACKGROUND: Breast cancer is the most prevalent malignancies among the women that have a high mortality. Previous studies demonstrated that hypericin, a bioactive component of Hypericum perforatum have a cytotoxic effect on the malignant cell lines. However, an anti-carcinogenic activity of hyperici...

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Autores principales: Mirmalek, Seyed Abbas, Azizi, Mohammad Amin, Jangholi, Ehsan, Yadollah-Damavandi, Soheila, Javidi, Mohammad Amin, Parsa, Yekta, Parsa, Tina, Salimi-Tabatabaee, Seyed Alireza, Ghasemzadeh kolagar, Hossein, Alizadeh-Navaei, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748624/
https://www.ncbi.nlm.nih.gov/pubmed/26865836
http://dx.doi.org/10.1186/s12935-016-0279-4
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author Mirmalek, Seyed Abbas
Azizi, Mohammad Amin
Jangholi, Ehsan
Yadollah-Damavandi, Soheila
Javidi, Mohammad Amin
Parsa, Yekta
Parsa, Tina
Salimi-Tabatabaee, Seyed Alireza
Ghasemzadeh kolagar, Hossein
Alizadeh-Navaei, Reza
author_facet Mirmalek, Seyed Abbas
Azizi, Mohammad Amin
Jangholi, Ehsan
Yadollah-Damavandi, Soheila
Javidi, Mohammad Amin
Parsa, Yekta
Parsa, Tina
Salimi-Tabatabaee, Seyed Alireza
Ghasemzadeh kolagar, Hossein
Alizadeh-Navaei, Reza
author_sort Mirmalek, Seyed Abbas
collection PubMed
description BACKGROUND: Breast cancer is the most prevalent malignancies among the women that have a high mortality. Previous studies demonstrated that hypericin, a bioactive component of Hypericum perforatum have a cytotoxic effect on the malignant cell lines. However, an anti-carcinogenic activity of hypericin on MCF-7 is uncertain. To investigate the cytotoxic effect of hypericin on MCF-7 cells, a human breast adenocarcinoma cell-line, that resistance to chemotherapy. METHODS: The MCF-7 and fibroblast (as normal cell line) were treated with various concentrations of hypericin, and Cisplatin as a positive control for 24 and 48 h. Cytotoxicity activity was measured and confirmed by MTT assay and Trypan blue staining, respectively. In addition, Apoptosis were determined by Annexin V/Propidium Iodide assay. Immunocytochemistry (ICC) analysis for bcl2 and p53 proteins performed to further investigate different expression of these genes in different samples. RESULTS: Both cisplatin and the hypericin exhibited a dose-dependent cytotoxic effect in the MCF-7 cell line. Although the LD50 of the hypericin was significantly lower when compared to cispaltin (5 vs. 20 μg/ml), it continued to decrease the growth rate of the MCF-7 cells when tested at higher concentration than LD50. In contrast, cisplatine, at higher concentration than LD50, completely inhibited the growth of the MCF-7 in 48 h. Regarding Annexin V/Propidium results, treatment of MCF-7 cells with LD50 concentration of cisplatin and hypericin showed 60 and 52 % apoptosis in 24 h, respectively. ICC analysis for bcl2 and p53 also confirmed our results; in treated samples for the dose of LD50 in 24 and 48 h of cisplatin and hypercin, more cells expressed p53 (guardian of cells in front of tumor formation/progression) and less expressed bcl2 (which has anti apoptotic activity) compared to untreated samples. CONCLUSIONS: Considering that hypericin showed to be cytotoxic, it seems to be a chemopreventive agent and a good candidate for antineoplastic drug development.
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spelling pubmed-47486242016-02-11 Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line Mirmalek, Seyed Abbas Azizi, Mohammad Amin Jangholi, Ehsan Yadollah-Damavandi, Soheila Javidi, Mohammad Amin Parsa, Yekta Parsa, Tina Salimi-Tabatabaee, Seyed Alireza Ghasemzadeh kolagar, Hossein Alizadeh-Navaei, Reza Cancer Cell Int Primary Research BACKGROUND: Breast cancer is the most prevalent malignancies among the women that have a high mortality. Previous studies demonstrated that hypericin, a bioactive component of Hypericum perforatum have a cytotoxic effect on the malignant cell lines. However, an anti-carcinogenic activity of hypericin on MCF-7 is uncertain. To investigate the cytotoxic effect of hypericin on MCF-7 cells, a human breast adenocarcinoma cell-line, that resistance to chemotherapy. METHODS: The MCF-7 and fibroblast (as normal cell line) were treated with various concentrations of hypericin, and Cisplatin as a positive control for 24 and 48 h. Cytotoxicity activity was measured and confirmed by MTT assay and Trypan blue staining, respectively. In addition, Apoptosis were determined by Annexin V/Propidium Iodide assay. Immunocytochemistry (ICC) analysis for bcl2 and p53 proteins performed to further investigate different expression of these genes in different samples. RESULTS: Both cisplatin and the hypericin exhibited a dose-dependent cytotoxic effect in the MCF-7 cell line. Although the LD50 of the hypericin was significantly lower when compared to cispaltin (5 vs. 20 μg/ml), it continued to decrease the growth rate of the MCF-7 cells when tested at higher concentration than LD50. In contrast, cisplatine, at higher concentration than LD50, completely inhibited the growth of the MCF-7 in 48 h. Regarding Annexin V/Propidium results, treatment of MCF-7 cells with LD50 concentration of cisplatin and hypericin showed 60 and 52 % apoptosis in 24 h, respectively. ICC analysis for bcl2 and p53 also confirmed our results; in treated samples for the dose of LD50 in 24 and 48 h of cisplatin and hypercin, more cells expressed p53 (guardian of cells in front of tumor formation/progression) and less expressed bcl2 (which has anti apoptotic activity) compared to untreated samples. CONCLUSIONS: Considering that hypericin showed to be cytotoxic, it seems to be a chemopreventive agent and a good candidate for antineoplastic drug development. BioMed Central 2016-02-09 /pmc/articles/PMC4748624/ /pubmed/26865836 http://dx.doi.org/10.1186/s12935-016-0279-4 Text en © Mirmalek et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Primary Research
Mirmalek, Seyed Abbas
Azizi, Mohammad Amin
Jangholi, Ehsan
Yadollah-Damavandi, Soheila
Javidi, Mohammad Amin
Parsa, Yekta
Parsa, Tina
Salimi-Tabatabaee, Seyed Alireza
Ghasemzadeh kolagar, Hossein
Alizadeh-Navaei, Reza
Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
title Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
title_full Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
title_fullStr Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
title_full_unstemmed Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
title_short Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
title_sort cytotoxic and apoptogenic effect of hypericin, the bioactive component of hypericum perforatum on the mcf-7 human breast cancer cell line
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748624/
https://www.ncbi.nlm.nih.gov/pubmed/26865836
http://dx.doi.org/10.1186/s12935-016-0279-4
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