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Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions

tRNA is essential for translation and decoding of the proteome. The yeast proteome responds to stress and tRNA biosynthesis contributes in this response by repression of tRNA transcription and alterations of tRNA modification. Here we report that the stress response also involves processing of pre-t...

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Autores principales: Foretek, Dominika, Wu, Jingyan, Hopper, Anita K., Boguta, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748812/
https://www.ncbi.nlm.nih.gov/pubmed/26729922
http://dx.doi.org/10.1261/rna.054973.115
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author Foretek, Dominika
Wu, Jingyan
Hopper, Anita K.
Boguta, Magdalena
author_facet Foretek, Dominika
Wu, Jingyan
Hopper, Anita K.
Boguta, Magdalena
author_sort Foretek, Dominika
collection PubMed
description tRNA is essential for translation and decoding of the proteome. The yeast proteome responds to stress and tRNA biosynthesis contributes in this response by repression of tRNA transcription and alterations of tRNA modification. Here we report that the stress response also involves processing of pre-tRNA 3′ termini. By a combination of Northern analyses and RNA sequencing, we show that upon shift to elevated temperatures and/or to glycerol-containing medium, aberrant pre-tRNAs accumulate in yeast cells. For pre-tRNA(UAU)(Ile) and pre-tRNA(UUU)(Lys) these aberrant forms are unprocessed at the 5′ ends, but they possess extended 3′ termini. Sequencing analyses showed that partial 3′ processing precedes 5′ processing for pre-tRNA(UAU)(Ile). An aberrant pre-tRNA(Tyr) that accumulates also possesses extended 3′ termini, but it is processed at the 5′ terminus. Similar forms of these aberrant pre-tRNAs are detected in the rex1Δ strain that is defective in 3′ exonucleolytic trimming of pre-tRNAs but are absent in the lhp1Δ mutant lacking 3′ end protection. We further show direct correlation between the inhibition of 3′ end processing rate and the stringency of growth conditions. Moreover, under stress conditions Rex1 nuclease seems to be limiting for 3′ end processing, by decreased availability linked to increased protection by Lhp1. Thus, our data document complex 3′ processing that is inhibited by stress in a tRNA-type and condition-specific manner. This stress-responsive tRNA 3′ end maturation process presumably contributes to fine-tune the levels of functional tRNA in budding yeast in response to environmental conditions.
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spelling pubmed-47488122017-03-01 Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions Foretek, Dominika Wu, Jingyan Hopper, Anita K. Boguta, Magdalena RNA Article tRNA is essential for translation and decoding of the proteome. The yeast proteome responds to stress and tRNA biosynthesis contributes in this response by repression of tRNA transcription and alterations of tRNA modification. Here we report that the stress response also involves processing of pre-tRNA 3′ termini. By a combination of Northern analyses and RNA sequencing, we show that upon shift to elevated temperatures and/or to glycerol-containing medium, aberrant pre-tRNAs accumulate in yeast cells. For pre-tRNA(UAU)(Ile) and pre-tRNA(UUU)(Lys) these aberrant forms are unprocessed at the 5′ ends, but they possess extended 3′ termini. Sequencing analyses showed that partial 3′ processing precedes 5′ processing for pre-tRNA(UAU)(Ile). An aberrant pre-tRNA(Tyr) that accumulates also possesses extended 3′ termini, but it is processed at the 5′ terminus. Similar forms of these aberrant pre-tRNAs are detected in the rex1Δ strain that is defective in 3′ exonucleolytic trimming of pre-tRNAs but are absent in the lhp1Δ mutant lacking 3′ end protection. We further show direct correlation between the inhibition of 3′ end processing rate and the stringency of growth conditions. Moreover, under stress conditions Rex1 nuclease seems to be limiting for 3′ end processing, by decreased availability linked to increased protection by Lhp1. Thus, our data document complex 3′ processing that is inhibited by stress in a tRNA-type and condition-specific manner. This stress-responsive tRNA 3′ end maturation process presumably contributes to fine-tune the levels of functional tRNA in budding yeast in response to environmental conditions. Cold Spring Harbor Laboratory Press 2016-03 /pmc/articles/PMC4748812/ /pubmed/26729922 http://dx.doi.org/10.1261/rna.054973.115 Text en © 2016 Foretek et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Article
Foretek, Dominika
Wu, Jingyan
Hopper, Anita K.
Boguta, Magdalena
Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions
title Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions
title_full Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions
title_fullStr Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions
title_full_unstemmed Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions
title_short Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions
title_sort control of saccharomyces cerevisiae pre-trna processing by environmental conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748812/
https://www.ncbi.nlm.nih.gov/pubmed/26729922
http://dx.doi.org/10.1261/rna.054973.115
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