Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells

Zeaxanthin at nonlethal dosages (3–10 μM) significantly inhibited the cell migration of cultured uveal melanoma cells (C918 cell line) as determined by wound healing assay and Boyden chamber assay. Matrigel invasion assay showed that cell invasion of uveal melanoma cells could be significantly inhib...

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Autores principales: Bi, Ming-Chao, Hose, Nicole, Xu, Cai-Lian, Zhang, Chen, Sassoon, Jodi, Song, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749803/
https://www.ncbi.nlm.nih.gov/pubmed/26942004
http://dx.doi.org/10.1155/2016/8734309
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author Bi, Ming-Chao
Hose, Nicole
Xu, Cai-Lian
Zhang, Chen
Sassoon, Jodi
Song, E.
author_facet Bi, Ming-Chao
Hose, Nicole
Xu, Cai-Lian
Zhang, Chen
Sassoon, Jodi
Song, E.
author_sort Bi, Ming-Chao
collection PubMed
description Zeaxanthin at nonlethal dosages (3–10 μM) significantly inhibited the cell migration of cultured uveal melanoma cells (C918 cell line) as determined by wound healing assay and Boyden chamber assay. Matrigel invasion assay showed that cell invasion of uveal melanoma cells could be significantly inhibited by zeaxanthin. Secretion of MMP-2 by melanoma cells was significantly inhibited by zeaxanthin in a dose-dependent manner as measured by ELISA kit. Zeaxanthin also significantly inhibited the NF-κB levels in nuclear extracts of the UM cells, which is the upstream of the MMP-2 secretion. These results suggest that zeaxanthin might be a potentially therapeutic approach in the prevention of metastasis in uveal melanoma.
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spelling pubmed-47498032016-03-03 Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells Bi, Ming-Chao Hose, Nicole Xu, Cai-Lian Zhang, Chen Sassoon, Jodi Song, E. J Ophthalmol Research Article Zeaxanthin at nonlethal dosages (3–10 μM) significantly inhibited the cell migration of cultured uveal melanoma cells (C918 cell line) as determined by wound healing assay and Boyden chamber assay. Matrigel invasion assay showed that cell invasion of uveal melanoma cells could be significantly inhibited by zeaxanthin. Secretion of MMP-2 by melanoma cells was significantly inhibited by zeaxanthin in a dose-dependent manner as measured by ELISA kit. Zeaxanthin also significantly inhibited the NF-κB levels in nuclear extracts of the UM cells, which is the upstream of the MMP-2 secretion. These results suggest that zeaxanthin might be a potentially therapeutic approach in the prevention of metastasis in uveal melanoma. Hindawi Publishing Corporation 2016 2016-01-28 /pmc/articles/PMC4749803/ /pubmed/26942004 http://dx.doi.org/10.1155/2016/8734309 Text en Copyright © 2016 Ming-Chao Bi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bi, Ming-Chao
Hose, Nicole
Xu, Cai-Lian
Zhang, Chen
Sassoon, Jodi
Song, E.
Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells
title Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells
title_full Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells
title_fullStr Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells
title_full_unstemmed Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells
title_short Nonlethal Levels of Zeaxanthin Inhibit Cell Migration, Invasion, and Secretion of MMP-2 via NF-κB Pathway in Cultured Human Uveal Melanoma Cells
title_sort nonlethal levels of zeaxanthin inhibit cell migration, invasion, and secretion of mmp-2 via nf-κb pathway in cultured human uveal melanoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749803/
https://www.ncbi.nlm.nih.gov/pubmed/26942004
http://dx.doi.org/10.1155/2016/8734309
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