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Polymorphisms in NOS3, MTHFR, APOB and TNF-α Genes and Risk of Coronary Atherosclerotic Lesions in Iranian Patients

BACKGROUND: Atherosclerosis is a complex multifocal arterial disease involving interactions between multiple genetic and environmental factors. OBJECTIVES: In the present study, we investigated the possible association between NOS3 (rs1799983), MTHFR (rs1801133), APOB (rs5742904) and TNF-α (rs361525...

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Detalles Bibliográficos
Autores principales: Heidari, Mohammad Mehdi, Khatami, Mehri, Hadadzadeh, Mehdi, Kazemi, Mahbobeh, Mahamed, Sahar, Malekzadeh, Pegah, Mirjalili, Massomeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749848/
https://www.ncbi.nlm.nih.gov/pubmed/26878010
http://dx.doi.org/10.5812/cardiovascmed.29134
Descripción
Sumario:BACKGROUND: Atherosclerosis is a complex multifocal arterial disease involving interactions between multiple genetic and environmental factors. OBJECTIVES: In the present study, we investigated the possible association between NOS3 (rs1799983), MTHFR (rs1801133), APOB (rs5742904) and TNF-α (rs361525) polymorphisms and the risk of coronary atherosclerotic lesions in Iranian patients. PATIENTS AND METHODS: In the case-control study, 108 patients with coronary atherosclerosis disease and 95 control subjects with no family history of cardiovascular disease were enrolled. Genotypes for NOS3, MTHFR, APOB and TNF-α polymorphisms were identified using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). RESULTS: We specifically detected the NOS3 TT genotype in 12 patients (11.11%) and did not find the same genotype in any of the controls. The frequencies of T allele in patients and the controls were 24% and 17.8%, respectively. The prevalence of the MTHFR TT genotype was 16.7% in patients and 2.2% in control groups. The prevalence of the APOB-100 (R3500Q) mutation in this patient population was 0%. The frequency of the A allele in the TNF-α gene was 11.1% and 11% in patients and controls, respectively, and the AA genotype was undetected. CONCLUSIONS: Our results show a significant association of NOS3 and MTHFR gene polymorphisms with coronary atherosclerotic lesions. Therefore, these variants might influence the risk of coronary artery disease, specifically in the Iranian population.