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Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters
It is increasingly apparent that nature evolved peroxiredoxins not only as H(2)O(2) scavengers but also as highly sensitive H(2)O(2) sensors and signal transducers. Here we ask whether the H(2)O(2) sensing role of Prx can be exploited to develop probes that allow to monitor intracellular H(2)O(2) le...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Molecular and Cellular Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749873/ https://www.ncbi.nlm.nih.gov/pubmed/26810074 http://dx.doi.org/10.14348/molcells.2016.2328 |
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author | Van Laer, Koen Dick, Tobias P. |
author_facet | Van Laer, Koen Dick, Tobias P. |
author_sort | Van Laer, Koen |
collection | PubMed |
description | It is increasingly apparent that nature evolved peroxiredoxins not only as H(2)O(2) scavengers but also as highly sensitive H(2)O(2) sensors and signal transducers. Here we ask whether the H(2)O(2) sensing role of Prx can be exploited to develop probes that allow to monitor intracellular H(2)O(2) levels with unprecedented sensitivity. Indeed, simple gel shift assays visualizing the oxidation of endogenous 2-Cys peroxiredoxins have already been used to detect subtle changes in intracellular H(2)O(2) concentration. The challenge however is to create a genetically encoded probe that offers real-time measurements of H(2)O(2) levels in intact cells via the Prx oxidation state. We discuss potential design strategies for Prx-based probes based on either the redox-sensitive fluorophore roGFP or the conformation-sensitive fluorophore cpYFP. Furthermore, we outline the structural and chemical complexities which need to be addressed when using Prx as a sensing moiety for H(2)O(2) probes. We suggest experimental strategies to investigate the influence of these complexities on probe behavior. In doing so, we hope to stimulate the development of Prx-based probes which may spearhead the further study of cellular H(2)O(2) homeostasis and Prx signaling. |
format | Online Article Text |
id | pubmed-4749873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Korean Society for Molecular and Cellular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-47498732016-02-25 Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters Van Laer, Koen Dick, Tobias P. Mol Cells Minireview It is increasingly apparent that nature evolved peroxiredoxins not only as H(2)O(2) scavengers but also as highly sensitive H(2)O(2) sensors and signal transducers. Here we ask whether the H(2)O(2) sensing role of Prx can be exploited to develop probes that allow to monitor intracellular H(2)O(2) levels with unprecedented sensitivity. Indeed, simple gel shift assays visualizing the oxidation of endogenous 2-Cys peroxiredoxins have already been used to detect subtle changes in intracellular H(2)O(2) concentration. The challenge however is to create a genetically encoded probe that offers real-time measurements of H(2)O(2) levels in intact cells via the Prx oxidation state. We discuss potential design strategies for Prx-based probes based on either the redox-sensitive fluorophore roGFP or the conformation-sensitive fluorophore cpYFP. Furthermore, we outline the structural and chemical complexities which need to be addressed when using Prx as a sensing moiety for H(2)O(2) probes. We suggest experimental strategies to investigate the influence of these complexities on probe behavior. In doing so, we hope to stimulate the development of Prx-based probes which may spearhead the further study of cellular H(2)O(2) homeostasis and Prx signaling. Korean Society for Molecular and Cellular Biology 2016-01-31 2016-01-25 /pmc/articles/PMC4749873/ /pubmed/26810074 http://dx.doi.org/10.14348/molcells.2016.2328 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Minireview Van Laer, Koen Dick, Tobias P. Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters |
title | Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters |
title_full | Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters |
title_fullStr | Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters |
title_full_unstemmed | Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters |
title_short | Utilizing Natural and Engineered Peroxiredoxins As Intracellular Peroxide Reporters |
title_sort | utilizing natural and engineered peroxiredoxins as intracellular peroxide reporters |
topic | Minireview |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749873/ https://www.ncbi.nlm.nih.gov/pubmed/26810074 http://dx.doi.org/10.14348/molcells.2016.2328 |
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