Cargando…

Proteomic analysis of SETD6 interacting proteins

SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyelob...

Descripción completa

Detalles Bibliográficos
Autores principales: Cohn, Ofir, Chen, Ayelet, Feldman, Michal, Levy, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749940/
https://www.ncbi.nlm.nih.gov/pubmed/26937450
http://dx.doi.org/10.1016/j.dib.2016.01.042
_version_ 1782415344463249408
author Cohn, Ofir
Chen, Ayelet
Feldman, Michal
Levy, Dan
author_facet Cohn, Ofir
Chen, Ayelet
Feldman, Michal
Levy, Dan
author_sort Cohn, Ofir
collection PubMed
description SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding.
format Online
Article
Text
id pubmed-4749940
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-47499402016-03-02 Proteomic analysis of SETD6 interacting proteins Cohn, Ofir Chen, Ayelet Feldman, Michal Levy, Dan Data Brief Data Article SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding. Elsevier 2016-01-29 /pmc/articles/PMC4749940/ /pubmed/26937450 http://dx.doi.org/10.1016/j.dib.2016.01.042 Text en © 2016 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Cohn, Ofir
Chen, Ayelet
Feldman, Michal
Levy, Dan
Proteomic analysis of SETD6 interacting proteins
title Proteomic analysis of SETD6 interacting proteins
title_full Proteomic analysis of SETD6 interacting proteins
title_fullStr Proteomic analysis of SETD6 interacting proteins
title_full_unstemmed Proteomic analysis of SETD6 interacting proteins
title_short Proteomic analysis of SETD6 interacting proteins
title_sort proteomic analysis of setd6 interacting proteins
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749940/
https://www.ncbi.nlm.nih.gov/pubmed/26937450
http://dx.doi.org/10.1016/j.dib.2016.01.042
work_keys_str_mv AT cohnofir proteomicanalysisofsetd6interactingproteins
AT chenayelet proteomicanalysisofsetd6interactingproteins
AT feldmanmichal proteomicanalysisofsetd6interactingproteins
AT levydan proteomicanalysisofsetd6interactingproteins