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Proteomic analysis of SETD6 interacting proteins
SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyelob...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749940/ https://www.ncbi.nlm.nih.gov/pubmed/26937450 http://dx.doi.org/10.1016/j.dib.2016.01.042 |
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author | Cohn, Ofir Chen, Ayelet Feldman, Michal Levy, Dan |
author_facet | Cohn, Ofir Chen, Ayelet Feldman, Michal Levy, Dan |
author_sort | Cohn, Ofir |
collection | PubMed |
description | SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding. |
format | Online Article Text |
id | pubmed-4749940 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-47499402016-03-02 Proteomic analysis of SETD6 interacting proteins Cohn, Ofir Chen, Ayelet Feldman, Michal Levy, Dan Data Brief Data Article SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding. Elsevier 2016-01-29 /pmc/articles/PMC4749940/ /pubmed/26937450 http://dx.doi.org/10.1016/j.dib.2016.01.042 Text en © 2016 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Cohn, Ofir Chen, Ayelet Feldman, Michal Levy, Dan Proteomic analysis of SETD6 interacting proteins |
title | Proteomic analysis of SETD6 interacting proteins |
title_full | Proteomic analysis of SETD6 interacting proteins |
title_fullStr | Proteomic analysis of SETD6 interacting proteins |
title_full_unstemmed | Proteomic analysis of SETD6 interacting proteins |
title_short | Proteomic analysis of SETD6 interacting proteins |
title_sort | proteomic analysis of setd6 interacting proteins |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749940/ https://www.ncbi.nlm.nih.gov/pubmed/26937450 http://dx.doi.org/10.1016/j.dib.2016.01.042 |
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