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Histological and transcriptome analyses of testes from Duroc and Meishan boars
Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for his...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749976/ https://www.ncbi.nlm.nih.gov/pubmed/26865000 http://dx.doi.org/10.1038/srep20758 |
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author | Ding, Haisheng Luo, Yan Liu, Min Huang, Jingshu Xu, Dequan |
author_facet | Ding, Haisheng Luo, Yan Liu, Min Huang, Jingshu Xu, Dequan |
author_sort | Ding, Haisheng |
collection | PubMed |
description | Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and transcriptome analyses. Haematoxylin-eosin staining was conducted to observe histological structure of the testes in Duroc and Meishan boars at different ages. Although spermatogenesis occurred prior to 75 days in Meishan boars, the number of spermatogonia and Sertoli cells in Meishan boars were less than in Duroc boars at adulthood. The diameters of the seminiferous tubules of the testes differed significantly during the initiation of development of the seminiferous tubules between the two breeds. We obtained differentially expressed functional genes and analysed seven pathways involved in male sexual maturity and spermatogenesis using RNA-seq. We also detected four main alternative splicing events and many single nucleotide polymorphisms from testes. Eight functionally important genes were validated by qPCR, and Neurotrophin 3 was subjected to quantification and cellular localization analysis. Our study provides the first transcriptome evidence for the differences in sexual function development between Meishan and Duroc boars. |
format | Online Article Text |
id | pubmed-4749976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47499762016-02-17 Histological and transcriptome analyses of testes from Duroc and Meishan boars Ding, Haisheng Luo, Yan Liu, Min Huang, Jingshu Xu, Dequan Sci Rep Article Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and transcriptome analyses. Haematoxylin-eosin staining was conducted to observe histological structure of the testes in Duroc and Meishan boars at different ages. Although spermatogenesis occurred prior to 75 days in Meishan boars, the number of spermatogonia and Sertoli cells in Meishan boars were less than in Duroc boars at adulthood. The diameters of the seminiferous tubules of the testes differed significantly during the initiation of development of the seminiferous tubules between the two breeds. We obtained differentially expressed functional genes and analysed seven pathways involved in male sexual maturity and spermatogenesis using RNA-seq. We also detected four main alternative splicing events and many single nucleotide polymorphisms from testes. Eight functionally important genes were validated by qPCR, and Neurotrophin 3 was subjected to quantification and cellular localization analysis. Our study provides the first transcriptome evidence for the differences in sexual function development between Meishan and Duroc boars. Nature Publishing Group 2016-02-11 /pmc/articles/PMC4749976/ /pubmed/26865000 http://dx.doi.org/10.1038/srep20758 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ding, Haisheng Luo, Yan Liu, Min Huang, Jingshu Xu, Dequan Histological and transcriptome analyses of testes from Duroc and Meishan boars |
title | Histological and transcriptome analyses of testes from Duroc and Meishan boars |
title_full | Histological and transcriptome analyses of testes from Duroc and Meishan boars |
title_fullStr | Histological and transcriptome analyses of testes from Duroc and Meishan boars |
title_full_unstemmed | Histological and transcriptome analyses of testes from Duroc and Meishan boars |
title_short | Histological and transcriptome analyses of testes from Duroc and Meishan boars |
title_sort | histological and transcriptome analyses of testes from duroc and meishan boars |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749976/ https://www.ncbi.nlm.nih.gov/pubmed/26865000 http://dx.doi.org/10.1038/srep20758 |
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