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Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies

We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of targe...

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Detalles Bibliográficos
Autores principales:	Kellner, Nikola, Schwarz, Johannes, Sturm, Miriam, Fernandez-Martinez, Javier, Griesel, Sabine, Zhang, Wenzhu, Chait, Brian T., Rout, Michael P., Kück, Ulrich, Hurt, Ed
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750058/ https://www.ncbi.nlm.nih.gov/pubmed/26864114 http://dx.doi.org/10.1038/srep20937

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