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Epigenetic Classification of Human Mesenchymal Stromal Cells
Standardization of mesenchymal stromal cells (MSCs) is hampered by the lack of a precise definition for these cell preparations; for example, there are no molecular markers to discern MSCs and fibroblasts. In this study, we followed the hypothesis that specific DNA methylation (DNAm) patterns can as...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750140/ https://www.ncbi.nlm.nih.gov/pubmed/26862701 http://dx.doi.org/10.1016/j.stemcr.2016.01.003 |
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author | de Almeida, Danilo Candido Ferreira, Marcelo R.P. Franzen, Julia Weidner, Carola I. Frobel, Joana Zenke, Martin Costa, Ivan G. Wagner, Wolfgang |
author_facet | de Almeida, Danilo Candido Ferreira, Marcelo R.P. Franzen, Julia Weidner, Carola I. Frobel, Joana Zenke, Martin Costa, Ivan G. Wagner, Wolfgang |
author_sort | de Almeida, Danilo Candido |
collection | PubMed |
description | Standardization of mesenchymal stromal cells (MSCs) is hampered by the lack of a precise definition for these cell preparations; for example, there are no molecular markers to discern MSCs and fibroblasts. In this study, we followed the hypothesis that specific DNA methylation (DNAm) patterns can assist classification of MSCs. We utilized 190 DNAm profiles to address the impact of tissue of origin, donor age, replicative senescence, and serum supplements on the epigenetic makeup. Based on this, we elaborated a simple epigenetic signature based on two CpG sites to classify MSCs and fibroblasts, referred to as the Epi-MSC-Score. Another two-CpG signature can distinguish between MSCs from bone marrow and adipose tissue, referred to as the Epi-Tissue-Score. These assays were validated by site-specific pyrosequencing analysis in 34 primary cell preparations. Furthermore, even individual subclones of MSCs were correctly classified by our epigenetic signatures. In summary, we propose an alternative concept to use DNAm patterns for molecular definition of cell preparations, and our epigenetic scores facilitate robust and cost-effective quality control of MSC cultures. |
format | Online Article Text |
id | pubmed-4750140 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-47501402016-03-02 Epigenetic Classification of Human Mesenchymal Stromal Cells de Almeida, Danilo Candido Ferreira, Marcelo R.P. Franzen, Julia Weidner, Carola I. Frobel, Joana Zenke, Martin Costa, Ivan G. Wagner, Wolfgang Stem Cell Reports Report Standardization of mesenchymal stromal cells (MSCs) is hampered by the lack of a precise definition for these cell preparations; for example, there are no molecular markers to discern MSCs and fibroblasts. In this study, we followed the hypothesis that specific DNA methylation (DNAm) patterns can assist classification of MSCs. We utilized 190 DNAm profiles to address the impact of tissue of origin, donor age, replicative senescence, and serum supplements on the epigenetic makeup. Based on this, we elaborated a simple epigenetic signature based on two CpG sites to classify MSCs and fibroblasts, referred to as the Epi-MSC-Score. Another two-CpG signature can distinguish between MSCs from bone marrow and adipose tissue, referred to as the Epi-Tissue-Score. These assays were validated by site-specific pyrosequencing analysis in 34 primary cell preparations. Furthermore, even individual subclones of MSCs were correctly classified by our epigenetic signatures. In summary, we propose an alternative concept to use DNAm patterns for molecular definition of cell preparations, and our epigenetic scores facilitate robust and cost-effective quality control of MSC cultures. Elsevier 2016-02-09 /pmc/articles/PMC4750140/ /pubmed/26862701 http://dx.doi.org/10.1016/j.stemcr.2016.01.003 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Report de Almeida, Danilo Candido Ferreira, Marcelo R.P. Franzen, Julia Weidner, Carola I. Frobel, Joana Zenke, Martin Costa, Ivan G. Wagner, Wolfgang Epigenetic Classification of Human Mesenchymal Stromal Cells |
title | Epigenetic Classification of Human Mesenchymal Stromal Cells |
title_full | Epigenetic Classification of Human Mesenchymal Stromal Cells |
title_fullStr | Epigenetic Classification of Human Mesenchymal Stromal Cells |
title_full_unstemmed | Epigenetic Classification of Human Mesenchymal Stromal Cells |
title_short | Epigenetic Classification of Human Mesenchymal Stromal Cells |
title_sort | epigenetic classification of human mesenchymal stromal cells |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750140/ https://www.ncbi.nlm.nih.gov/pubmed/26862701 http://dx.doi.org/10.1016/j.stemcr.2016.01.003 |
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