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Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection
Here, we assessed outcome of experimental infection by Neospora caninum in goats intravenously inoculated with 10(6) tachyzoites of the Nc-Spain7 isolate at 40 (G1), 90 (G2) and 120 (G3) days of gestation. Infected goats had fever between 5 and 9 days post inoculation (dpi); all were seropositive at...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750177/ https://www.ncbi.nlm.nih.gov/pubmed/26864744 http://dx.doi.org/10.1186/s13567-016-0312-6 |
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author | Porto, Wagnner José Nascimento Regidor-Cerrillo, Javier de Cássia Peixoto Kim, Pomy Benavides, Julio dos Santos Silva, Ana Clécia Horcajo, Pilar da Fonseca Oliveira, Andrea Alice Ferre, Ignacio Mota, Rinaldo Aparecido Ortega-Mora, Luis Miguel |
author_facet | Porto, Wagnner José Nascimento Regidor-Cerrillo, Javier de Cássia Peixoto Kim, Pomy Benavides, Julio dos Santos Silva, Ana Clécia Horcajo, Pilar da Fonseca Oliveira, Andrea Alice Ferre, Ignacio Mota, Rinaldo Aparecido Ortega-Mora, Luis Miguel |
author_sort | Porto, Wagnner José Nascimento |
collection | PubMed |
description | Here, we assessed outcome of experimental infection by Neospora caninum in goats intravenously inoculated with 10(6) tachyzoites of the Nc-Spain7 isolate at 40 (G1), 90 (G2) and 120 (G3) days of gestation. Infected goats had fever between 5 and 9 days post inoculation (dpi); all were seropositive at the time of abortion/birth. Foetal death occurred in G1 from 10 to 21 dpi (n = 7) and in G2 from 27 to 35 dpi (n = 4). Goats in G2 also had seropositive stillbirth (n = 1) and healthy kids (n = 2). G3 goats (n = 7) had 3 seropositive and 3 seronegative weak kids, and 2 seronegative healthy kids. Parasite DNA detection in placentomes was 100% in G2, 85.7% in G3 and in G1 was detected only in placentomes from the goats with foetal losses from 17 dpi (100%). Parasites were detected in foetal/kid brain (>85.7%) and liver (≥50%) of G2 and G3, and in G1 after 17 dpi (100%). The highest parasite loads were detected in the placentomes of G1 from 17 dpi and G2, and in foetal tissues of G1 from 17 dpi and G3. Multifocal necrotic lesions were observed in the placentas of the three groups, but they were larger and more frequent in G1 and G2. Similar lesions were observed in foetal tissues, but they were more frequent in G3. These findings suggest that, as observed in cattle and sheep, the clinical consequences of N. caninum in pregnant goats are dependent in part on the time of gestation when animals were infected. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-016-0312-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4750177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47501772016-02-12 Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection Porto, Wagnner José Nascimento Regidor-Cerrillo, Javier de Cássia Peixoto Kim, Pomy Benavides, Julio dos Santos Silva, Ana Clécia Horcajo, Pilar da Fonseca Oliveira, Andrea Alice Ferre, Ignacio Mota, Rinaldo Aparecido Ortega-Mora, Luis Miguel Vet Res Research Article Here, we assessed outcome of experimental infection by Neospora caninum in goats intravenously inoculated with 10(6) tachyzoites of the Nc-Spain7 isolate at 40 (G1), 90 (G2) and 120 (G3) days of gestation. Infected goats had fever between 5 and 9 days post inoculation (dpi); all were seropositive at the time of abortion/birth. Foetal death occurred in G1 from 10 to 21 dpi (n = 7) and in G2 from 27 to 35 dpi (n = 4). Goats in G2 also had seropositive stillbirth (n = 1) and healthy kids (n = 2). G3 goats (n = 7) had 3 seropositive and 3 seronegative weak kids, and 2 seronegative healthy kids. Parasite DNA detection in placentomes was 100% in G2, 85.7% in G3 and in G1 was detected only in placentomes from the goats with foetal losses from 17 dpi (100%). Parasites were detected in foetal/kid brain (>85.7%) and liver (≥50%) of G2 and G3, and in G1 after 17 dpi (100%). The highest parasite loads were detected in the placentomes of G1 from 17 dpi and G2, and in foetal tissues of G1 from 17 dpi and G3. Multifocal necrotic lesions were observed in the placentas of the three groups, but they were larger and more frequent in G1 and G2. Similar lesions were observed in foetal tissues, but they were more frequent in G3. These findings suggest that, as observed in cattle and sheep, the clinical consequences of N. caninum in pregnant goats are dependent in part on the time of gestation when animals were infected. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-016-0312-6) contains supplementary material, which is available to authorized users. BioMed Central 2016-02-11 2016 /pmc/articles/PMC4750177/ /pubmed/26864744 http://dx.doi.org/10.1186/s13567-016-0312-6 Text en © Porto et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Porto, Wagnner José Nascimento Regidor-Cerrillo, Javier de Cássia Peixoto Kim, Pomy Benavides, Julio dos Santos Silva, Ana Clécia Horcajo, Pilar da Fonseca Oliveira, Andrea Alice Ferre, Ignacio Mota, Rinaldo Aparecido Ortega-Mora, Luis Miguel Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection |
title | Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection |
title_full | Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection |
title_fullStr | Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection |
title_full_unstemmed | Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection |
title_short | Experimental caprine neosporosis: the influence of gestational stage on the outcome of infection |
title_sort | experimental caprine neosporosis: the influence of gestational stage on the outcome of infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750177/ https://www.ncbi.nlm.nih.gov/pubmed/26864744 http://dx.doi.org/10.1186/s13567-016-0312-6 |
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