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Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2
BACKGROUND: Propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. In this study we aimed to investigate: i) the antiviral activities of Hatay propolis samples against HSV-1 and HSV-2 in HEp-2 cell line, and ii) the presence of the synergistic effects...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750782/ https://www.ncbi.nlm.nih.gov/pubmed/26856414 http://dx.doi.org/10.12659/MSM.897282 |
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author | Yildirim, Ayse Duran, Gulay Gulbol Duran, Nizami Jenedi, Kemal Bolgul, Behiye Sezgin Miraloglu, Meral Muz, Mustafa |
author_facet | Yildirim, Ayse Duran, Gulay Gulbol Duran, Nizami Jenedi, Kemal Bolgul, Behiye Sezgin Miraloglu, Meral Muz, Mustafa |
author_sort | Yildirim, Ayse |
collection | PubMed |
description | BACKGROUND: Propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. In this study we aimed to investigate: i) the antiviral activities of Hatay propolis samples against HSV-1 and HSV-2 in HEp-2 cell line, and ii) the presence of the synergistic effects of propolis with acyclovir against these viruses. MATERIAL/METHODS: All experiments were carried out in HEp-2 cell cultures. Proliferation assays were performed in 24-well flat bottom microplates. We inoculated 1×10(5) cells per ml and RPMI 1640 medium with 10% fetal calf serum into each well. Studies to determine cytotoxic effect were performed. To investigate the presence of antiviral activity of propolis samples, different concentrations of propolis (3200, 1600, 800, 400, 200, 100, 75, 50, and 25 μg/mL) were added into the culture medium. The amplifications of HSV-1 and HSV-2 DNA were performed by real-time PCR method. Acyclovir (Sigma, USA) was chosen as a positive control. Cell morphology was evaluated by scanning electron microscopy (SEM). RESULTS: The replication of HSV-1 and HSV-2 was significantly suppressed in the presence of 25, 50, and 100 μg/mL of Hatay propolis. We found that propolis began to inhibit HSV-1 replication after 24 h of incubation and propolis activity against HSV-2 was found to start at 48 h following incubation. The activity of propolis against both HSV-1 and HSV-2 was confirmed by a significant decrease in the number of viral copies. CONCLUSIONS: We determined that Hatay propolis samples have important antiviral effects compared with acyclovir. In particular, the synergy produced by antiviral activity of propolis and acyclovir combined had a stronger effect against HSV-1 and HSV-2 than acyclovir alone. |
format | Online Article Text |
id | pubmed-4750782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47507822016-03-08 Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2 Yildirim, Ayse Duran, Gulay Gulbol Duran, Nizami Jenedi, Kemal Bolgul, Behiye Sezgin Miraloglu, Meral Muz, Mustafa Med Sci Monit Lab/In vitro Research BACKGROUND: Propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. In this study we aimed to investigate: i) the antiviral activities of Hatay propolis samples against HSV-1 and HSV-2 in HEp-2 cell line, and ii) the presence of the synergistic effects of propolis with acyclovir against these viruses. MATERIAL/METHODS: All experiments were carried out in HEp-2 cell cultures. Proliferation assays were performed in 24-well flat bottom microplates. We inoculated 1×10(5) cells per ml and RPMI 1640 medium with 10% fetal calf serum into each well. Studies to determine cytotoxic effect were performed. To investigate the presence of antiviral activity of propolis samples, different concentrations of propolis (3200, 1600, 800, 400, 200, 100, 75, 50, and 25 μg/mL) were added into the culture medium. The amplifications of HSV-1 and HSV-2 DNA were performed by real-time PCR method. Acyclovir (Sigma, USA) was chosen as a positive control. Cell morphology was evaluated by scanning electron microscopy (SEM). RESULTS: The replication of HSV-1 and HSV-2 was significantly suppressed in the presence of 25, 50, and 100 μg/mL of Hatay propolis. We found that propolis began to inhibit HSV-1 replication after 24 h of incubation and propolis activity against HSV-2 was found to start at 48 h following incubation. The activity of propolis against both HSV-1 and HSV-2 was confirmed by a significant decrease in the number of viral copies. CONCLUSIONS: We determined that Hatay propolis samples have important antiviral effects compared with acyclovir. In particular, the synergy produced by antiviral activity of propolis and acyclovir combined had a stronger effect against HSV-1 and HSV-2 than acyclovir alone. International Scientific Literature, Inc. 2016-02-09 /pmc/articles/PMC4750782/ /pubmed/26856414 http://dx.doi.org/10.12659/MSM.897282 Text en © Med Sci Monit, 2016 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License |
spellingShingle | Lab/In vitro Research Yildirim, Ayse Duran, Gulay Gulbol Duran, Nizami Jenedi, Kemal Bolgul, Behiye Sezgin Miraloglu, Meral Muz, Mustafa Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2 |
title | Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2 |
title_full | Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2 |
title_fullStr | Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2 |
title_full_unstemmed | Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2 |
title_short | Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2 |
title_sort | antiviral activity of hatay propolis against replication of herpes simplex virus type 1 and type 2 |
topic | Lab/In vitro Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750782/ https://www.ncbi.nlm.nih.gov/pubmed/26856414 http://dx.doi.org/10.12659/MSM.897282 |
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