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Genomic Organization of Human Transcription Initiation Complexes
A repertoire of transcription initiation factors engage the core promoter of mRNA genes to recruit RNA polymerase (Pol) II to initiate transcription, yet their precise spatial organization remains unclear. Using ChIP-exo, here we detail the interactions and genomic organization of initiation factors...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750860/ https://www.ncbi.nlm.nih.gov/pubmed/26866362 http://dx.doi.org/10.1371/journal.pone.0149339 |
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author | Pugh, B. Franklin Venters, Bryan J. |
author_facet | Pugh, B. Franklin Venters, Bryan J. |
author_sort | Pugh, B. Franklin |
collection | PubMed |
description | A repertoire of transcription initiation factors engage the core promoter of mRNA genes to recruit RNA polymerase (Pol) II to initiate transcription, yet their precise spatial organization remains unclear. Using ChIP-exo, here we detail the interactions and genomic organization of initiation factors TBP, TFIIB, and Pol II at mRNA genes and within CpG islands. We find that when Pol II moves into a transcriptionally paused state, TBP/TFIIB remain at the promoter. We show that TBP and TFIIB bound to the core promoter at two separate, resolvable locations that coincided with sites of divergent transcription initiation. We also examine the precise binding of TBP at Pol III transcribed tRNA genes. We find that TBP crosslinked to tRNA genes in a similar manner as at Pol II transcribed genes. This comprehensive and high resolution genome-wide detection of the initiation machinery produces a consolidated view of transcription initiation events humans at Pol II coding and Pol III transcribed tRNA genes. |
format | Online Article Text |
id | pubmed-4750860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47508602016-02-26 Genomic Organization of Human Transcription Initiation Complexes Pugh, B. Franklin Venters, Bryan J. PLoS One Research Article A repertoire of transcription initiation factors engage the core promoter of mRNA genes to recruit RNA polymerase (Pol) II to initiate transcription, yet their precise spatial organization remains unclear. Using ChIP-exo, here we detail the interactions and genomic organization of initiation factors TBP, TFIIB, and Pol II at mRNA genes and within CpG islands. We find that when Pol II moves into a transcriptionally paused state, TBP/TFIIB remain at the promoter. We show that TBP and TFIIB bound to the core promoter at two separate, resolvable locations that coincided with sites of divergent transcription initiation. We also examine the precise binding of TBP at Pol III transcribed tRNA genes. We find that TBP crosslinked to tRNA genes in a similar manner as at Pol II transcribed genes. This comprehensive and high resolution genome-wide detection of the initiation machinery produces a consolidated view of transcription initiation events humans at Pol II coding and Pol III transcribed tRNA genes. Public Library of Science 2016-02-11 /pmc/articles/PMC4750860/ /pubmed/26866362 http://dx.doi.org/10.1371/journal.pone.0149339 Text en © 2016 Pugh, Venters http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Pugh, B. Franklin Venters, Bryan J. Genomic Organization of Human Transcription Initiation Complexes |
title | Genomic Organization of Human Transcription Initiation Complexes |
title_full | Genomic Organization of Human Transcription Initiation Complexes |
title_fullStr | Genomic Organization of Human Transcription Initiation Complexes |
title_full_unstemmed | Genomic Organization of Human Transcription Initiation Complexes |
title_short | Genomic Organization of Human Transcription Initiation Complexes |
title_sort | genomic organization of human transcription initiation complexes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750860/ https://www.ncbi.nlm.nih.gov/pubmed/26866362 http://dx.doi.org/10.1371/journal.pone.0149339 |
work_keys_str_mv | AT pughbfranklin genomicorganizationofhumantranscriptioninitiationcomplexes AT ventersbryanj genomicorganizationofhumantranscriptioninitiationcomplexes |