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Evidence for new C-terminally truncated variants of α- and β-tubulins

Cellular α-tubulin can bear various carboxy-terminal sequences: full-length tubulin arising from gene neosynthesis is tyrosinated, and two truncated variants, corresponding to detyrosinated and Δ2 α‑tubulin, result from the sequential cleavage of one or two C-terminal residues, respectively. Here, b...

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Autores principales: Aillaud, Chrystelle, Bosc, Christophe, Saoudi, Yasmina, Denarier, Eric, Peris, Leticia, Sago, Laila, Taulet, Nicolas, Cieren, Adeline, Tort, Olivia, Magiera, Maria M., Janke, Carsten, Redeker, Virginie, Andrieux, Annie, Moutin, Marie-Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750924/
https://www.ncbi.nlm.nih.gov/pubmed/26739754
http://dx.doi.org/10.1091/mbc.E15-03-0137
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author Aillaud, Chrystelle
Bosc, Christophe
Saoudi, Yasmina
Denarier, Eric
Peris, Leticia
Sago, Laila
Taulet, Nicolas
Cieren, Adeline
Tort, Olivia
Magiera, Maria M.
Janke, Carsten
Redeker, Virginie
Andrieux, Annie
Moutin, Marie-Jo
author_facet Aillaud, Chrystelle
Bosc, Christophe
Saoudi, Yasmina
Denarier, Eric
Peris, Leticia
Sago, Laila
Taulet, Nicolas
Cieren, Adeline
Tort, Olivia
Magiera, Maria M.
Janke, Carsten
Redeker, Virginie
Andrieux, Annie
Moutin, Marie-Jo
author_sort Aillaud, Chrystelle
collection PubMed
description Cellular α-tubulin can bear various carboxy-terminal sequences: full-length tubulin arising from gene neosynthesis is tyrosinated, and two truncated variants, corresponding to detyrosinated and Δ2 α‑tubulin, result from the sequential cleavage of one or two C-terminal residues, respectively. Here, by using a novel antibody named 3EG that is highly specific to the –EEEG C-terminal sequence, we demonstrate the occurrence in neuronal tissues of a new αΔ3‑tubulin variant corresponding to α1A/B‑tubulin deleted of its last three residues (EEY). αΔ3‑tubulin has a specific distribution pattern: its quantity in the brain is similar to that of αΔ2-tubulin around birth but is much lower in adult tissue. This truncated α1A/B-tubulin variant can be generated from αΔ2-tubulin by the deglutamylases CCP1, CCP4, CCP5, and CCP6 but not by CCP2 and CCP3. Moreover, using 3EG antibody, we identify a C‑terminally truncated β-tubulin form with the same –EEEG C-terminal sequence. Using mass spectrometry, we demonstrate that β2A/B-tubulin is modified by truncation of the four C-terminal residues (EDEA). We show that this newly identified βΔ4-tubulin is ubiquitously present in cells and tissues and that its level is constant throughout the cell cycle. These new C-terminally truncated α- and β-tubulin variants, both ending with –EEEG sequence, are expected to regulate microtubule physiology. Of interest, the αΔ3-tubulin seems to be related to dynamic microtubules, resembling tyrosinated-tubulin rather than the other truncated variants, and may have critical function(s) in neuronal development.
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spelling pubmed-47509242016-04-30 Evidence for new C-terminally truncated variants of α- and β-tubulins Aillaud, Chrystelle Bosc, Christophe Saoudi, Yasmina Denarier, Eric Peris, Leticia Sago, Laila Taulet, Nicolas Cieren, Adeline Tort, Olivia Magiera, Maria M. Janke, Carsten Redeker, Virginie Andrieux, Annie Moutin, Marie-Jo Mol Biol Cell Articles Cellular α-tubulin can bear various carboxy-terminal sequences: full-length tubulin arising from gene neosynthesis is tyrosinated, and two truncated variants, corresponding to detyrosinated and Δ2 α‑tubulin, result from the sequential cleavage of one or two C-terminal residues, respectively. Here, by using a novel antibody named 3EG that is highly specific to the –EEEG C-terminal sequence, we demonstrate the occurrence in neuronal tissues of a new αΔ3‑tubulin variant corresponding to α1A/B‑tubulin deleted of its last three residues (EEY). αΔ3‑tubulin has a specific distribution pattern: its quantity in the brain is similar to that of αΔ2-tubulin around birth but is much lower in adult tissue. This truncated α1A/B-tubulin variant can be generated from αΔ2-tubulin by the deglutamylases CCP1, CCP4, CCP5, and CCP6 but not by CCP2 and CCP3. Moreover, using 3EG antibody, we identify a C‑terminally truncated β-tubulin form with the same –EEEG C-terminal sequence. Using mass spectrometry, we demonstrate that β2A/B-tubulin is modified by truncation of the four C-terminal residues (EDEA). We show that this newly identified βΔ4-tubulin is ubiquitously present in cells and tissues and that its level is constant throughout the cell cycle. These new C-terminally truncated α- and β-tubulin variants, both ending with –EEEG sequence, are expected to regulate microtubule physiology. Of interest, the αΔ3-tubulin seems to be related to dynamic microtubules, resembling tyrosinated-tubulin rather than the other truncated variants, and may have critical function(s) in neuronal development. The American Society for Cell Biology 2016-02-15 /pmc/articles/PMC4750924/ /pubmed/26739754 http://dx.doi.org/10.1091/mbc.E15-03-0137 Text en © 2016 Aillaud et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Aillaud, Chrystelle
Bosc, Christophe
Saoudi, Yasmina
Denarier, Eric
Peris, Leticia
Sago, Laila
Taulet, Nicolas
Cieren, Adeline
Tort, Olivia
Magiera, Maria M.
Janke, Carsten
Redeker, Virginie
Andrieux, Annie
Moutin, Marie-Jo
Evidence for new C-terminally truncated variants of α- and β-tubulins
title Evidence for new C-terminally truncated variants of α- and β-tubulins
title_full Evidence for new C-terminally truncated variants of α- and β-tubulins
title_fullStr Evidence for new C-terminally truncated variants of α- and β-tubulins
title_full_unstemmed Evidence for new C-terminally truncated variants of α- and β-tubulins
title_short Evidence for new C-terminally truncated variants of α- and β-tubulins
title_sort evidence for new c-terminally truncated variants of α- and β-tubulins
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750924/
https://www.ncbi.nlm.nih.gov/pubmed/26739754
http://dx.doi.org/10.1091/mbc.E15-03-0137
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