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A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions

Salmonella enterica serovar Weltevreden (S. Weltevreden) is an emerging cause of diarrheal and invasive disease in humans residing in tropical regions. Despite the regional and international emergence of this Salmonella serovar, relatively little is known about its genetic diversity, genomics or vir...

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Autores principales: Makendi, Carine, Page, Andrew J., Wren, Brendan W., Le Thi Phuong, Tu, Clare, Simon, Hale, Christine, Goulding, David, Klemm, Elizabeth J., Pickard, Derek, Okoro, Chinyere, Hunt, Martin, Thompson, Corinne N., Phu Huong Lan, Nguyen, Tran Do Hoang, Nhu, Thwaites, Guy E., Le Hello, Simon, Brisabois, Anne, Weill, François-Xavier, Baker, Stephen, Dougan, Gordon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750946/
https://www.ncbi.nlm.nih.gov/pubmed/26867150
http://dx.doi.org/10.1371/journal.pntd.0004446
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author Makendi, Carine
Page, Andrew J.
Wren, Brendan W.
Le Thi Phuong, Tu
Clare, Simon
Hale, Christine
Goulding, David
Klemm, Elizabeth J.
Pickard, Derek
Okoro, Chinyere
Hunt, Martin
Thompson, Corinne N.
Phu Huong Lan, Nguyen
Tran Do Hoang, Nhu
Thwaites, Guy E.
Le Hello, Simon
Brisabois, Anne
Weill, François-Xavier
Baker, Stephen
Dougan, Gordon
author_facet Makendi, Carine
Page, Andrew J.
Wren, Brendan W.
Le Thi Phuong, Tu
Clare, Simon
Hale, Christine
Goulding, David
Klemm, Elizabeth J.
Pickard, Derek
Okoro, Chinyere
Hunt, Martin
Thompson, Corinne N.
Phu Huong Lan, Nguyen
Tran Do Hoang, Nhu
Thwaites, Guy E.
Le Hello, Simon
Brisabois, Anne
Weill, François-Xavier
Baker, Stephen
Dougan, Gordon
author_sort Makendi, Carine
collection PubMed
description Salmonella enterica serovar Weltevreden (S. Weltevreden) is an emerging cause of diarrheal and invasive disease in humans residing in tropical regions. Despite the regional and international emergence of this Salmonella serovar, relatively little is known about its genetic diversity, genomics or virulence potential in model systems. Here we used whole genome sequencing and bioinformatics analyses to define the phylogenetic structure of a diverse global selection of S. Weltevreden. Phylogenetic analysis of more than 100 isolates demonstrated that the population of S. Weltevreden can be segregated into two main phylogenetic clusters, one associated predominantly with continental Southeast Asia and the other more internationally dispersed. Subcluster analysis suggested the local evolution of S. Weltevreden within specific geographical regions. Four of the isolates were sequenced using long read sequencing to produce high quality reference genomes. Phenotypic analysis in Hep-2 cells and in a murine infection model indicated that S. Weltevreden were significantly attenuated in these models compared to the classical S. Typhimurium reference strain SL1344. Our work outlines novel insights into this important emerging pathogen and provides a baseline understanding for future research studies.
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spelling pubmed-47509462016-02-26 A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions Makendi, Carine Page, Andrew J. Wren, Brendan W. Le Thi Phuong, Tu Clare, Simon Hale, Christine Goulding, David Klemm, Elizabeth J. Pickard, Derek Okoro, Chinyere Hunt, Martin Thompson, Corinne N. Phu Huong Lan, Nguyen Tran Do Hoang, Nhu Thwaites, Guy E. Le Hello, Simon Brisabois, Anne Weill, François-Xavier Baker, Stephen Dougan, Gordon PLoS Negl Trop Dis Research Article Salmonella enterica serovar Weltevreden (S. Weltevreden) is an emerging cause of diarrheal and invasive disease in humans residing in tropical regions. Despite the regional and international emergence of this Salmonella serovar, relatively little is known about its genetic diversity, genomics or virulence potential in model systems. Here we used whole genome sequencing and bioinformatics analyses to define the phylogenetic structure of a diverse global selection of S. Weltevreden. Phylogenetic analysis of more than 100 isolates demonstrated that the population of S. Weltevreden can be segregated into two main phylogenetic clusters, one associated predominantly with continental Southeast Asia and the other more internationally dispersed. Subcluster analysis suggested the local evolution of S. Weltevreden within specific geographical regions. Four of the isolates were sequenced using long read sequencing to produce high quality reference genomes. Phenotypic analysis in Hep-2 cells and in a murine infection model indicated that S. Weltevreden were significantly attenuated in these models compared to the classical S. Typhimurium reference strain SL1344. Our work outlines novel insights into this important emerging pathogen and provides a baseline understanding for future research studies. Public Library of Science 2016-02-11 /pmc/articles/PMC4750946/ /pubmed/26867150 http://dx.doi.org/10.1371/journal.pntd.0004446 Text en © 2016 Makendi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Makendi, Carine
Page, Andrew J.
Wren, Brendan W.
Le Thi Phuong, Tu
Clare, Simon
Hale, Christine
Goulding, David
Klemm, Elizabeth J.
Pickard, Derek
Okoro, Chinyere
Hunt, Martin
Thompson, Corinne N.
Phu Huong Lan, Nguyen
Tran Do Hoang, Nhu
Thwaites, Guy E.
Le Hello, Simon
Brisabois, Anne
Weill, François-Xavier
Baker, Stephen
Dougan, Gordon
A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions
title A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions
title_full A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions
title_fullStr A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions
title_full_unstemmed A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions
title_short A Phylogenetic and Phenotypic Analysis of Salmonella enterica Serovar Weltevreden, an Emerging Agent of Diarrheal Disease in Tropical Regions
title_sort phylogenetic and phenotypic analysis of salmonella enterica serovar weltevreden, an emerging agent of diarrheal disease in tropical regions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750946/
https://www.ncbi.nlm.nih.gov/pubmed/26867150
http://dx.doi.org/10.1371/journal.pntd.0004446
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