Cargando…

Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan

In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs) isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido, Japan, were genetically analyzed to understand r...

Descripción completa

Detalles Bibliográficos
Autores principales: ABE, Yuri, TAMURA, Tomokazu, TORII, Shiho, WAKAMORI, Shiho, NAGAI, Makoto, MITSUHASHI, Kazuya, MINE, Junki, FUJIMOTO, Yuri, NAGASHIMA, Naofumi, YOSHINO, Fumi, SUGITA, Yukihiko, NOMURA, Takushi, OKAMATSU, Masatoshi, KIDA, Hiroshi, SAKODA, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751118/
https://www.ncbi.nlm.nih.gov/pubmed/26400674
http://dx.doi.org/10.1292/jvms.15-0186
_version_ 1782415530913693696
author ABE, Yuri
TAMURA, Tomokazu
TORII, Shiho
WAKAMORI, Shiho
NAGAI, Makoto
MITSUHASHI, Kazuya
MINE, Junki
FUJIMOTO, Yuri
NAGASHIMA, Naofumi
YOSHINO, Fumi
SUGITA, Yukihiko
NOMURA, Takushi
OKAMATSU, Masatoshi
KIDA, Hiroshi
SAKODA, Yoshihiro
author_facet ABE, Yuri
TAMURA, Tomokazu
TORII, Shiho
WAKAMORI, Shiho
NAGAI, Makoto
MITSUHASHI, Kazuya
MINE, Junki
FUJIMOTO, Yuri
NAGASHIMA, Naofumi
YOSHINO, Fumi
SUGITA, Yukihiko
NOMURA, Takushi
OKAMATSU, Masatoshi
KIDA, Hiroshi
SAKODA, Yoshihiro
author_sort ABE, Yuri
collection PubMed
description In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs) isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido, Japan, were genetically analyzed to understand recent epidemics. Phylogenetic analysis based on nucleotide sequences of the 5′-untranslated region of viral genome revealed that 766 isolates were classified as genotype 1 (BVDV-1; 544 isolates) and genotype 2 (BVDV-2; 222). BVDV-1 isolates were further divided into BVDV-1a (93), 1b (371) and 1c (80) subgenotypes, and all BVDV-2 isolates were grouped into BVDV-2a subgenotype (222). Further comparative analysis was performed with BVDV-1a, 1b and 2a viruses isolated from 2001 to 2014. Phylogenetic analysis based on nucleotide sequences of the viral glycoprotein E2 gene, a major target of neutralizing antibodies, revealed that BVDV-1a, 1b and 2a isolates were further classified into several clusters. Cross-neutralization tests showed that BVDV-1b isolates were antigenically different from BVDV-1a isolates, and almost BVDV-1a, 1b and 2a isolates were antigenically similar among each subgenotype and each E2 cluster. Taken together, BVDV-1b viruses are still predominant, and BVDV-2a viruses have increased recently in Hokkaido, Japan. Field isolates of BVDV-1a, 1b and 2a show genetic diversity on the E2 gene with antigenic conservation among each subgenotype during the last 14 years.
format Online
Article
Text
id pubmed-4751118
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-47511182016-02-26 Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan ABE, Yuri TAMURA, Tomokazu TORII, Shiho WAKAMORI, Shiho NAGAI, Makoto MITSUHASHI, Kazuya MINE, Junki FUJIMOTO, Yuri NAGASHIMA, Naofumi YOSHINO, Fumi SUGITA, Yukihiko NOMURA, Takushi OKAMATSU, Masatoshi KIDA, Hiroshi SAKODA, Yoshihiro J Vet Med Sci Virology In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs) isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido, Japan, were genetically analyzed to understand recent epidemics. Phylogenetic analysis based on nucleotide sequences of the 5′-untranslated region of viral genome revealed that 766 isolates were classified as genotype 1 (BVDV-1; 544 isolates) and genotype 2 (BVDV-2; 222). BVDV-1 isolates were further divided into BVDV-1a (93), 1b (371) and 1c (80) subgenotypes, and all BVDV-2 isolates were grouped into BVDV-2a subgenotype (222). Further comparative analysis was performed with BVDV-1a, 1b and 2a viruses isolated from 2001 to 2014. Phylogenetic analysis based on nucleotide sequences of the viral glycoprotein E2 gene, a major target of neutralizing antibodies, revealed that BVDV-1a, 1b and 2a isolates were further classified into several clusters. Cross-neutralization tests showed that BVDV-1b isolates were antigenically different from BVDV-1a isolates, and almost BVDV-1a, 1b and 2a isolates were antigenically similar among each subgenotype and each E2 cluster. Taken together, BVDV-1b viruses are still predominant, and BVDV-2a viruses have increased recently in Hokkaido, Japan. Field isolates of BVDV-1a, 1b and 2a show genetic diversity on the E2 gene with antigenic conservation among each subgenotype during the last 14 years. The Japanese Society of Veterinary Science 2015-09-01 2016-01 /pmc/articles/PMC4751118/ /pubmed/26400674 http://dx.doi.org/10.1292/jvms.15-0186 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
ABE, Yuri
TAMURA, Tomokazu
TORII, Shiho
WAKAMORI, Shiho
NAGAI, Makoto
MITSUHASHI, Kazuya
MINE, Junki
FUJIMOTO, Yuri
NAGASHIMA, Naofumi
YOSHINO, Fumi
SUGITA, Yukihiko
NOMURA, Takushi
OKAMATSU, Masatoshi
KIDA, Hiroshi
SAKODA, Yoshihiro
Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan
title Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan
title_full Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan
title_fullStr Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan
title_full_unstemmed Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan
title_short Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan
title_sort genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in hokkaido, japan
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751118/
https://www.ncbi.nlm.nih.gov/pubmed/26400674
http://dx.doi.org/10.1292/jvms.15-0186
work_keys_str_mv AT abeyuri geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT tamuratomokazu geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT toriishiho geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT wakamorishiho geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT nagaimakoto geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT mitsuhashikazuya geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT minejunki geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT fujimotoyuri geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT nagashimanaofumi geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT yoshinofumi geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT sugitayukihiko geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT nomuratakushi geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT okamatsumasatoshi geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT kidahiroshi geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan
AT sakodayoshihiro geneticandantigeniccharacterizationofbovineviraldiarrheavirusesisolatedfromcattleinhokkaidojapan