Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification

Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated t...

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Detalles Bibliográficos
Autores principales: KOIDE, Rie, SAKAGUCHI, Shoichi, OGAWA, Makoto, MIYAZAWA, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751125/
https://www.ncbi.nlm.nih.gov/pubmed/26268665
http://dx.doi.org/10.1292/jvms.15-0239
Descripción
Sumario:Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID(50))/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID(50) per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV.

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