Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification

Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated t...

Descripción completa

Detalles Bibliográficos
Autores principales: KOIDE, Rie, SAKAGUCHI, Shoichi, OGAWA, Makoto, MIYAZAWA, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751125/
https://www.ncbi.nlm.nih.gov/pubmed/26268665
http://dx.doi.org/10.1292/jvms.15-0239
_version_ 1782415532504383488
author KOIDE, Rie
SAKAGUCHI, Shoichi
OGAWA, Makoto
MIYAZAWA, Takayuki
author_facet KOIDE, Rie
SAKAGUCHI, Shoichi
OGAWA, Makoto
MIYAZAWA, Takayuki
author_sort KOIDE, Rie
collection PubMed
description Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID(50))/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID(50) per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV.
format Online
Article
Text
id pubmed-4751125
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-47511252016-02-26 Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification KOIDE, Rie SAKAGUCHI, Shoichi OGAWA, Makoto MIYAZAWA, Takayuki J Vet Med Sci Virology Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID(50))/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID(50) per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV. The Japanese Society of Veterinary Science 2015-08-11 2016-01 /pmc/articles/PMC4751125/ /pubmed/26268665 http://dx.doi.org/10.1292/jvms.15-0239 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
KOIDE, Rie
SAKAGUCHI, Shoichi
OGAWA, Makoto
MIYAZAWA, Takayuki
Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
title Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
title_full Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
title_fullStr Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
title_full_unstemmed Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
title_short Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
title_sort rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751125/
https://www.ncbi.nlm.nih.gov/pubmed/26268665
http://dx.doi.org/10.1292/jvms.15-0239
work_keys_str_mv AT koiderie rapiddetectionoffelinemorbillivirusbyareversetranscriptionloopmediatedisothermalamplification
AT sakaguchishoichi rapiddetectionoffelinemorbillivirusbyareversetranscriptionloopmediatedisothermalamplification
AT ogawamakoto rapiddetectionoffelinemorbillivirusbyareversetranscriptionloopmediatedisothermalamplification
AT miyazawatakayuki rapiddetectionoffelinemorbillivirusbyareversetranscriptionloopmediatedisothermalamplification

Ejemplares similares