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Development of a genotyping tool for a functionally relevant CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus macaques
In cynomolgus macaques, which are widely used in drug metabolism studies, CYP2C19 (formerly known as CYP2C75) is abundantly expressed in liver, metabolizes human CYP2C substrates and is thus an important drug-metabolizing enzyme. One of the cynomolgus CYP2C19 alleles (p.Phe100Asn, p.Ala103Val and p....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751135/ https://www.ncbi.nlm.nih.gov/pubmed/26300440 http://dx.doi.org/10.1292/jvms.15-0416 |
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author | UNO, Yasuhiro YAMAZAKI, Hiroshi |
author_facet | UNO, Yasuhiro YAMAZAKI, Hiroshi |
author_sort | UNO, Yasuhiro |
collection | PubMed |
description | In cynomolgus macaques, which are widely used in drug metabolism studies, CYP2C19 (formerly known as CYP2C75) is abundantly expressed in liver, metabolizes human CYP2C substrates and is thus an important drug-metabolizing enzyme. One of the cynomolgus CYP2C19 alleles (p.Phe100Asn, p.Ala103Val and p.Ile112Leu) results in substantially reduced metabolic activity and thus is an important allele in drug metabolism studies. For this allele, a genotyping tool was developed using allele-specific TaqMan probe. Genotyping 40 Cambodian cynomolgus macaques using this tool found 1 homozygote, 17 heterozygotes and 22 wild type animals, and the result was confirmed by direct-sequencing. Interestingly, this allele frequency was similar to that of Chinese cynomolgus macaques. The genotyping tool established is useful for drug metabolism studies using cynomolgus macaques. |
format | Online Article Text |
id | pubmed-4751135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47511352016-02-26 Development of a genotyping tool for a functionally relevant CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus macaques UNO, Yasuhiro YAMAZAKI, Hiroshi J Vet Med Sci Pharmacology In cynomolgus macaques, which are widely used in drug metabolism studies, CYP2C19 (formerly known as CYP2C75) is abundantly expressed in liver, metabolizes human CYP2C substrates and is thus an important drug-metabolizing enzyme. One of the cynomolgus CYP2C19 alleles (p.Phe100Asn, p.Ala103Val and p.Ile112Leu) results in substantially reduced metabolic activity and thus is an important allele in drug metabolism studies. For this allele, a genotyping tool was developed using allele-specific TaqMan probe. Genotyping 40 Cambodian cynomolgus macaques using this tool found 1 homozygote, 17 heterozygotes and 22 wild type animals, and the result was confirmed by direct-sequencing. Interestingly, this allele frequency was similar to that of Chinese cynomolgus macaques. The genotyping tool established is useful for drug metabolism studies using cynomolgus macaques. The Japanese Society of Veterinary Science 2015-08-21 2016-01 /pmc/articles/PMC4751135/ /pubmed/26300440 http://dx.doi.org/10.1292/jvms.15-0416 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Pharmacology UNO, Yasuhiro YAMAZAKI, Hiroshi Development of a genotyping tool for a functionally relevant CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus macaques |
title | Development of a genotyping tool for a functionally relevant
CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus
macaques |
title_full | Development of a genotyping tool for a functionally relevant
CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus
macaques |
title_fullStr | Development of a genotyping tool for a functionally relevant
CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus
macaques |
title_full_unstemmed | Development of a genotyping tool for a functionally relevant
CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus
macaques |
title_short | Development of a genotyping tool for a functionally relevant
CYP2C19 allele (Phe100Asn, Ala103Val and Ile112Leu) in cynomolgus
macaques |
title_sort | development of a genotyping tool for a functionally relevant
cyp2c19 allele (phe100asn, ala103val and ile112leu) in cynomolgus
macaques |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751135/ https://www.ncbi.nlm.nih.gov/pubmed/26300440 http://dx.doi.org/10.1292/jvms.15-0416 |
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