The requirement of environmental acidification for Ibaraki virus infection to host cells

The effect of environmental acidification on Ibaraki virus (IBAV) infection was tested using endosomal inhibitory chemicals and low pH treatment. Treatment of target cells with endosomal inhibitors significantly decreased the progeny virus production. IBAV outer capsid proteins, VP5 and VP2, were re...

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Autores principales: TSURUTA, Yuya, SHIBUTANI, Shusaku T., WATANABE, Rie, IWATA, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751137/
https://www.ncbi.nlm.nih.gov/pubmed/26321298
http://dx.doi.org/10.1292/jvms.15-0222
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author TSURUTA, Yuya
SHIBUTANI, Shusaku T.
WATANABE, Rie
IWATA, Hiroyuki
author_facet TSURUTA, Yuya
SHIBUTANI, Shusaku T.
WATANABE, Rie
IWATA, Hiroyuki
author_sort TSURUTA, Yuya
collection PubMed
description The effect of environmental acidification on Ibaraki virus (IBAV) infection was tested using endosomal inhibitory chemicals and low pH treatment. Treatment of target cells with endosomal inhibitors significantly decreased the progeny virus production. IBAV outer capsid proteins, VP5 and VP2, were removed from virion when purified IBAV was exposed to low pH environment. Further experiment showed that the exposure to low pH buffer facilitated IBAV infection when the cellular endosomal pathway was impaired by bafilomycin A1. Results obtained in this study suggest that acidic environment is essential to initiate IBAV infection.
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spelling pubmed-47511372016-02-26 The requirement of environmental acidification for Ibaraki virus infection to host cells TSURUTA, Yuya SHIBUTANI, Shusaku T. WATANABE, Rie IWATA, Hiroyuki J Vet Med Sci Virology The effect of environmental acidification on Ibaraki virus (IBAV) infection was tested using endosomal inhibitory chemicals and low pH treatment. Treatment of target cells with endosomal inhibitors significantly decreased the progeny virus production. IBAV outer capsid proteins, VP5 and VP2, were removed from virion when purified IBAV was exposed to low pH environment. Further experiment showed that the exposure to low pH buffer facilitated IBAV infection when the cellular endosomal pathway was impaired by bafilomycin A1. Results obtained in this study suggest that acidic environment is essential to initiate IBAV infection. The Japanese Society of Veterinary Science 2015-08-28 2016-01 /pmc/articles/PMC4751137/ /pubmed/26321298 http://dx.doi.org/10.1292/jvms.15-0222 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
TSURUTA, Yuya
SHIBUTANI, Shusaku T.
WATANABE, Rie
IWATA, Hiroyuki
The requirement of environmental acidification for Ibaraki virus infection to host cells
title The requirement of environmental acidification for Ibaraki virus infection to host cells
title_full The requirement of environmental acidification for Ibaraki virus infection to host cells
title_fullStr The requirement of environmental acidification for Ibaraki virus infection to host cells
title_full_unstemmed The requirement of environmental acidification for Ibaraki virus infection to host cells
title_short The requirement of environmental acidification for Ibaraki virus infection to host cells
title_sort requirement of environmental acidification for ibaraki virus infection to host cells
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751137/
https://www.ncbi.nlm.nih.gov/pubmed/26321298
http://dx.doi.org/10.1292/jvms.15-0222
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