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Regulation of functional KCNQ1OT1 lncRNA by β-catenin
Long noncoding RNAs (lncRNAs) have been implicated in many biological processes through epigenetic mechanisms. We previously reported that KCNQ1OT1, an imprinted antisense lncRNA in the human KCNQ1 locus on chromosome 11p15.5, is involved in cis-limited silencing within an imprinted KCNQ1 cluster. F...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751614/ https://www.ncbi.nlm.nih.gov/pubmed/26868975 http://dx.doi.org/10.1038/srep20690 |
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author | Sunamura, Naohiro Ohira, Takahito Kataoka, Miki Inaoka, Daigo Tanabe, Hideyuki Nakayama, Yuji Oshimura, Mitsuo Kugoh, Hiroyuki |
author_facet | Sunamura, Naohiro Ohira, Takahito Kataoka, Miki Inaoka, Daigo Tanabe, Hideyuki Nakayama, Yuji Oshimura, Mitsuo Kugoh, Hiroyuki |
author_sort | Sunamura, Naohiro |
collection | PubMed |
description | Long noncoding RNAs (lncRNAs) have been implicated in many biological processes through epigenetic mechanisms. We previously reported that KCNQ1OT1, an imprinted antisense lncRNA in the human KCNQ1 locus on chromosome 11p15.5, is involved in cis-limited silencing within an imprinted KCNQ1 cluster. Furthermore, aberration of KCNQ1OT1 transcription was observed with a high frequency in colorectal cancers. However, the molecular mechanism of the transcriptional regulation and the functional role of KCNQ1OT1 in colorectal cancer remain unclear. Here, we show that the KCNQ1OT1 transcriptional level was significantly increased in human colorectal cancer cells in which β-catenin was excessively accumulated in the nucleus. Additionally, overexpression of β-catenin resulted in an increase in KCNQ1OT1 lncRNA-coated territory. On the other hand, knockdown of β-catenin resulted in significant decrease of KCNQ1OT1 lncRNA-coated territory and an increase in the mRNA expression of the SLC22A18 and PHLDA2 genes that are regulated by KCNQ1OT1. We showed that β-catenin can promote KCNQ1OT1 transcription through direct binding to the KCNQ1OT1 promoter. Our evidence indicates that β-catenin signaling may contribute to development of colorectal cancer by functioning as a novel lncRNA regulatory factor via direct targeting of KCNQ1OT1. |
format | Online Article Text |
id | pubmed-4751614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47516142016-02-22 Regulation of functional KCNQ1OT1 lncRNA by β-catenin Sunamura, Naohiro Ohira, Takahito Kataoka, Miki Inaoka, Daigo Tanabe, Hideyuki Nakayama, Yuji Oshimura, Mitsuo Kugoh, Hiroyuki Sci Rep Article Long noncoding RNAs (lncRNAs) have been implicated in many biological processes through epigenetic mechanisms. We previously reported that KCNQ1OT1, an imprinted antisense lncRNA in the human KCNQ1 locus on chromosome 11p15.5, is involved in cis-limited silencing within an imprinted KCNQ1 cluster. Furthermore, aberration of KCNQ1OT1 transcription was observed with a high frequency in colorectal cancers. However, the molecular mechanism of the transcriptional regulation and the functional role of KCNQ1OT1 in colorectal cancer remain unclear. Here, we show that the KCNQ1OT1 transcriptional level was significantly increased in human colorectal cancer cells in which β-catenin was excessively accumulated in the nucleus. Additionally, overexpression of β-catenin resulted in an increase in KCNQ1OT1 lncRNA-coated territory. On the other hand, knockdown of β-catenin resulted in significant decrease of KCNQ1OT1 lncRNA-coated territory and an increase in the mRNA expression of the SLC22A18 and PHLDA2 genes that are regulated by KCNQ1OT1. We showed that β-catenin can promote KCNQ1OT1 transcription through direct binding to the KCNQ1OT1 promoter. Our evidence indicates that β-catenin signaling may contribute to development of colorectal cancer by functioning as a novel lncRNA regulatory factor via direct targeting of KCNQ1OT1. Nature Publishing Group 2016-02-12 /pmc/articles/PMC4751614/ /pubmed/26868975 http://dx.doi.org/10.1038/srep20690 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Sunamura, Naohiro Ohira, Takahito Kataoka, Miki Inaoka, Daigo Tanabe, Hideyuki Nakayama, Yuji Oshimura, Mitsuo Kugoh, Hiroyuki Regulation of functional KCNQ1OT1 lncRNA by β-catenin |
title | Regulation of functional KCNQ1OT1 lncRNA by β-catenin |
title_full | Regulation of functional KCNQ1OT1 lncRNA by β-catenin |
title_fullStr | Regulation of functional KCNQ1OT1 lncRNA by β-catenin |
title_full_unstemmed | Regulation of functional KCNQ1OT1 lncRNA by β-catenin |
title_short | Regulation of functional KCNQ1OT1 lncRNA by β-catenin |
title_sort | regulation of functional kcnq1ot1 lncrna by β-catenin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751614/ https://www.ncbi.nlm.nih.gov/pubmed/26868975 http://dx.doi.org/10.1038/srep20690 |
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