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miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria

MicroRNAs (miRNAs) are small regulatory RNAs that are involved in many biological process in eukaryotes. They play a crucial role in modulating genetic expression of their targets, which makes them integral components of transcriptional regulatory networks. As sponges (phylum Porifera) are commonly...

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Detalles Bibliográficos
Autores principales: Liew, Yi Jin, Ryu, Taewoo, Aranda, Manuel, Ravasi, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752309/
https://www.ncbi.nlm.nih.gov/pubmed/26871907
http://dx.doi.org/10.1371/journal.pone.0149080
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author Liew, Yi Jin
Ryu, Taewoo
Aranda, Manuel
Ravasi, Timothy
author_facet Liew, Yi Jin
Ryu, Taewoo
Aranda, Manuel
Ravasi, Timothy
author_sort Liew, Yi Jin
collection PubMed
description MicroRNAs (miRNAs) are small regulatory RNAs that are involved in many biological process in eukaryotes. They play a crucial role in modulating genetic expression of their targets, which makes them integral components of transcriptional regulatory networks. As sponges (phylum Porifera) are commonly considered the most basal metazoan, the in-depth capture of miRNAs from these organisms provides additional clues to the evolution of miRNA families in metazoans. Here, we identified the core proteins involved in the biogenesis of miRNAs, and obtained evidence for bona fide miRNA sequences for two marine sponges Stylissa carteri and Xestospongia testudinaria (11 and 19 respectively). Our analysis identified several miRNAs that are conserved amongst demosponges, and revealed that all of the novel miRNAs identified in these two species are specific to the class Demospongiae.
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spelling pubmed-47523092016-02-26 miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria Liew, Yi Jin Ryu, Taewoo Aranda, Manuel Ravasi, Timothy PLoS One Research Article MicroRNAs (miRNAs) are small regulatory RNAs that are involved in many biological process in eukaryotes. They play a crucial role in modulating genetic expression of their targets, which makes them integral components of transcriptional regulatory networks. As sponges (phylum Porifera) are commonly considered the most basal metazoan, the in-depth capture of miRNAs from these organisms provides additional clues to the evolution of miRNA families in metazoans. Here, we identified the core proteins involved in the biogenesis of miRNAs, and obtained evidence for bona fide miRNA sequences for two marine sponges Stylissa carteri and Xestospongia testudinaria (11 and 19 respectively). Our analysis identified several miRNAs that are conserved amongst demosponges, and revealed that all of the novel miRNAs identified in these two species are specific to the class Demospongiae. Public Library of Science 2016-02-12 /pmc/articles/PMC4752309/ /pubmed/26871907 http://dx.doi.org/10.1371/journal.pone.0149080 Text en © 2016 Liew et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liew, Yi Jin
Ryu, Taewoo
Aranda, Manuel
Ravasi, Timothy
miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
title miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
title_full miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
title_fullStr miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
title_full_unstemmed miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
title_short miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
title_sort mirna repertoires of demosponges stylissa carteri and xestospongia testudinaria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752309/
https://www.ncbi.nlm.nih.gov/pubmed/26871907
http://dx.doi.org/10.1371/journal.pone.0149080
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