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Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model

INTRODUCTION: Bone infections after trauma, i.e. posttraumatic osteomyelitis, pose one of the biggest problems of orthopedic surgery. Even after sufficient clinical therapy including vast debridement of infected bone and antibiotic treatment, regeneration of postinfectious bone seems to be restricte...

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Autores principales: Wagner, Johannes Maximilian, Zöllner, Hannah, Wallner, Christoph, Ismer, Britta, Schira, Jessica, Abraham, Stephanie, Harati, Kamran, Lehnhardt, Marcus, Behr, Björn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752466/
https://www.ncbi.nlm.nih.gov/pubmed/26872128
http://dx.doi.org/10.1371/journal.pone.0149389
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author Wagner, Johannes Maximilian
Zöllner, Hannah
Wallner, Christoph
Ismer, Britta
Schira, Jessica
Abraham, Stephanie
Harati, Kamran
Lehnhardt, Marcus
Behr, Björn
author_facet Wagner, Johannes Maximilian
Zöllner, Hannah
Wallner, Christoph
Ismer, Britta
Schira, Jessica
Abraham, Stephanie
Harati, Kamran
Lehnhardt, Marcus
Behr, Björn
author_sort Wagner, Johannes Maximilian
collection PubMed
description INTRODUCTION: Bone infections after trauma, i.e. posttraumatic osteomyelitis, pose one of the biggest problems of orthopedic surgery. Even after sufficient clinical therapy including vast debridement of infected bone and antibiotic treatment, regeneration of postinfectious bone seems to be restricted. One explanation includes the large sized defects resulting from sufficient debridement. Furthermore, it remains unclear if inflammatory processes after bone infection do affect bone regeneration. For continuing studies in this field, an animal model is needed where bone regeneration after sufficient treatment can be studied in detail. METHODS: For this purpose we created a stable infection in murine tibiae by Staphylococcus aureus inoculation. Thereafter, osteomyelitic bones were debrided thoroughly and animals were subsequently treated with antibiotics. Controls included debrided, non-infected, as well as infected animals exclusively treated with antibiotics. To verify sufficient treatment of infected bone, different assessments detecting S. aureus were utilized: agar plates, histology and RT-qPCR. RESULTS: All three detection methods revealed massive reduction or eradication of S. aureus within debrided bones 1 and 2 weeks postoperatively, whereas sole antibiotic therapy could not provide sufficient treatment of osteomyelitic bones. Debrided, previously infected bones showed significantly decreased bone formation, compared to debrided, non-infected controls. DISCUSSION: Thus, the animal model presented herein provides a reliable and fascinating tool to study posttraumatic osteomyelitis for clinical therapies.
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spelling pubmed-47524662016-02-26 Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model Wagner, Johannes Maximilian Zöllner, Hannah Wallner, Christoph Ismer, Britta Schira, Jessica Abraham, Stephanie Harati, Kamran Lehnhardt, Marcus Behr, Björn PLoS One Research Article INTRODUCTION: Bone infections after trauma, i.e. posttraumatic osteomyelitis, pose one of the biggest problems of orthopedic surgery. Even after sufficient clinical therapy including vast debridement of infected bone and antibiotic treatment, regeneration of postinfectious bone seems to be restricted. One explanation includes the large sized defects resulting from sufficient debridement. Furthermore, it remains unclear if inflammatory processes after bone infection do affect bone regeneration. For continuing studies in this field, an animal model is needed where bone regeneration after sufficient treatment can be studied in detail. METHODS: For this purpose we created a stable infection in murine tibiae by Staphylococcus aureus inoculation. Thereafter, osteomyelitic bones were debrided thoroughly and animals were subsequently treated with antibiotics. Controls included debrided, non-infected, as well as infected animals exclusively treated with antibiotics. To verify sufficient treatment of infected bone, different assessments detecting S. aureus were utilized: agar plates, histology and RT-qPCR. RESULTS: All three detection methods revealed massive reduction or eradication of S. aureus within debrided bones 1 and 2 weeks postoperatively, whereas sole antibiotic therapy could not provide sufficient treatment of osteomyelitic bones. Debrided, previously infected bones showed significantly decreased bone formation, compared to debrided, non-infected controls. DISCUSSION: Thus, the animal model presented herein provides a reliable and fascinating tool to study posttraumatic osteomyelitis for clinical therapies. Public Library of Science 2016-02-12 /pmc/articles/PMC4752466/ /pubmed/26872128 http://dx.doi.org/10.1371/journal.pone.0149389 Text en © 2016 Wagner et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wagner, Johannes Maximilian
Zöllner, Hannah
Wallner, Christoph
Ismer, Britta
Schira, Jessica
Abraham, Stephanie
Harati, Kamran
Lehnhardt, Marcus
Behr, Björn
Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model
title Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model
title_full Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model
title_fullStr Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model
title_full_unstemmed Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model
title_short Surgical Debridement Is Superior to Sole Antibiotic Therapy in a Novel Murine Posttraumatic Osteomyelitis Model
title_sort surgical debridement is superior to sole antibiotic therapy in a novel murine posttraumatic osteomyelitis model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752466/
https://www.ncbi.nlm.nih.gov/pubmed/26872128
http://dx.doi.org/10.1371/journal.pone.0149389
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