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A BioBrick™-Compatible Vector for Allelic Replacement Using the XylE Gene as Selection Marker
BACKGROUND: Circular plasmid-mediated homologous recombination is commonly used for marker-less allelic replacement, exploiting the endogenous recombination machinery of the host. Common limitations of existing methods include high false positive rates due to mutations in counter-selection genes, an...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752771/ https://www.ncbi.nlm.nih.gov/pubmed/26877712 http://dx.doi.org/10.1186/s12575-016-0036-z |