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Detection of genetic variation in sandalwood using various DNA markers
In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752954/ https://www.ncbi.nlm.nih.gov/pubmed/28330125 http://dx.doi.org/10.1007/s13205-016-0391-0 |
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author | Patel, Dimple M. Fougat, Ranbir S. Sakure, Amar A. Kumar, Sushil Kumar, Mukesh Mistry, Jigar G. |
author_facet | Patel, Dimple M. Fougat, Ranbir S. Sakure, Amar A. Kumar, Sushil Kumar, Mukesh Mistry, Jigar G. |
author_sort | Patel, Dimple M. |
collection | PubMed |
description | In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of 225, 181 (83.13 %) RAPD bands were polymorphic while out of 208, 156 (75.77 %) ISSR bands were polymorphic. The average polymorphism information content (PIC) for RAPD and ISSR was 0.84 and 0.86, respectively. A good correlation (0.96) was observed between the matrices produced by RAPD and ISSR primers. Though, there was high similarity among genotypes (0.79 for RAPD and 0.70 for ISSR), the observed genetic diversity was found good enough for the characterization of sandalwood genotypes. Cross-species transferability SSR markers developed in S. austrocaledonicum and S. insulare were found to be monomorphic. The results of the present investigation would provide valid guidelines for collection, conservation and characterization of sandalwood genetic resources. |
format | Online Article Text |
id | pubmed-4752954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-47529542016-02-16 Detection of genetic variation in sandalwood using various DNA markers Patel, Dimple M. Fougat, Ranbir S. Sakure, Amar A. Kumar, Sushil Kumar, Mukesh Mistry, Jigar G. 3 Biotech Original Article In the present study, 20 sandalwood (Santalum album L.) genotypes were characterized using RAPD, ISSR and SSR markers. Twenty-five RAPD and twenty-one ISSR primers that generated clear and reproducible banding patterns amplified 225 and 208 bands, respectively, among 20 sandalwood genotypes. Out of 225, 181 (83.13 %) RAPD bands were polymorphic while out of 208, 156 (75.77 %) ISSR bands were polymorphic. The average polymorphism information content (PIC) for RAPD and ISSR was 0.84 and 0.86, respectively. A good correlation (0.96) was observed between the matrices produced by RAPD and ISSR primers. Though, there was high similarity among genotypes (0.79 for RAPD and 0.70 for ISSR), the observed genetic diversity was found good enough for the characterization of sandalwood genotypes. Cross-species transferability SSR markers developed in S. austrocaledonicum and S. insulare were found to be monomorphic. The results of the present investigation would provide valid guidelines for collection, conservation and characterization of sandalwood genetic resources. Springer Berlin Heidelberg 2016-02-13 2016-06 /pmc/articles/PMC4752954/ /pubmed/28330125 http://dx.doi.org/10.1007/s13205-016-0391-0 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Patel, Dimple M. Fougat, Ranbir S. Sakure, Amar A. Kumar, Sushil Kumar, Mukesh Mistry, Jigar G. Detection of genetic variation in sandalwood using various DNA markers |
title | Detection of genetic variation in sandalwood using various DNA markers |
title_full | Detection of genetic variation in sandalwood using various DNA markers |
title_fullStr | Detection of genetic variation in sandalwood using various DNA markers |
title_full_unstemmed | Detection of genetic variation in sandalwood using various DNA markers |
title_short | Detection of genetic variation in sandalwood using various DNA markers |
title_sort | detection of genetic variation in sandalwood using various dna markers |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752954/ https://www.ncbi.nlm.nih.gov/pubmed/28330125 http://dx.doi.org/10.1007/s13205-016-0391-0 |
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